Technical datasheet
Description
The monoclonal antibody 6D8 recognizes the extracellular domain of human desmoglein-2. Desmogleins and desmocollins are members of the cadherin family of transmembrane proteins that together make up the core of the desmosome, a structure that provides transmembrane strength to tissues undergoing mechanical stress. The desmosomal cadherins, desmogleins and desmocollins, mediate calcium-dependent cell-cell adhesion by forming homotypic and heterotypic interactions with one another. The multiprotein desmosomal complex also includes the cytoplasmic desmosomal plaque proteins plakoglobin, phakophilins, and desmoplakin, which bind to the intracellular domain of the desmogleins and function to anchor the keratin intermediate filament network to site of cell–cell contacts.
In human, four desmogleins have been identified (Dsg1–4). Desmogleins are synthesized with a signal peptide that directs them to the endoplasmic reticulum and a proregion that is removed during protein processing. The mature protein includes four highly conserved extracellular domains (EC 1–4) and a fifth membrane proximal, more variable EC domain that is referred to as the “extracellular anchor domain. Desmoglein-2 is expressed on various cells including simple epithelia and myocardium, tumors and and many cell cultures.
Desmogleins play critical roles in cell adhesion and skin blistering diseases, as they are the target antigens of autoimmune antibodies and bacterial toxins. Desmosomal dysfunction has been implicated in a number of diseases, including striate palmoplantar keratoderma, skin fragility, and ectodermal dysplasia, and most recently arrhythmic right ventricular cardiomyopathy (ARVC).
Cross Reactivity
| Cross reactant |
Reactivity |
| Human desmoglein-1 |
No |
| Human desmoglein-3 |
No |
Immunogen
Extracellular EC4 domain
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG1
Application
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F5
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FC
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FS
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IA
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IF2,3, 7
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IP1,3-7
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P
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W1-3,5-7
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Yes
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No
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N.D.
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application Western has been tested by Hycult Biotech.
Application notes
W: A reduced sample treatment (Laemmli buffer) and SDS-PAGE was used. The band size is ~165 kDa (Ref.5).
IHC-F: Tissues frozen in OCT were fixed in 100% methanol for 15 min at 20_C, permeabilized in 50:50 methanol/acetone for 2 min at 20_C, and then treated with 1% Triton X-100 in PBS for 5 min at room temperature. Non-specific sites were blocked for 30min in blocking buffer (PBS/0.1% Triton X-100/1% BSA); (Ref.5).
Use
For immune fluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Aliases
Dsg2, Cadherin family member 5, HDGC
Positive control
A431 Cells
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
References
1. Wahl, J et al; Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J Cell Sci 1996, 109: 1143
2. Lewis, J et al; Cross-talk between adherens junctions and desmosomes depends on plakoglobin. J Cell Biol 1997, 136: 919
3. Wahl, J et al; Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 2002, 21: 37
4. Ota, T et al; No involvement of IgG autoantibodies against extracellular domains of desmoglein 2 in paraneoplastic pemphigus or inflammatory bowel diseases. J Dermatol Sci 2003, 32: 137
5. Mahoney, M et al; Delineation of diversified desmoglein distribution in stratified squamous epithelia: implications in diseases. Exp Dermatol 2006, 15: 101
6. Nava, P et al; Desmoglein-2: a novel regulator of apoptosis in the intestinal epithelium. MBC 2007, 18: 4565
7. Keim, S et al; Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2. Hybridoma 2008, 27: 249.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Also available
|
HM2112
|
Beta-Catenin, Human, mAb 9F2
|
|
HM2113
|
Desmoglein-1, Human, mAb 27B2
|
|
HM2115
|
Desmoglein-3, Human, mAb 5G11
|
|
HM2116
|
Plakoglobin, Human, mAb 15F11
|
|
HM2118
|
Alpha-Catenin, Human, mAb 1G5
|
References
1. Wahl, J et al; Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J Cell Sci 1996, 109: 1143
2. Lewis, J et al; Cross-talk between adherens junctions and desmosomes depends on plakoglobin. J Cell Biol 1997, 136: 919
3. Wahl, J et al; Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 2002, 21: 37
4. Ota, T et al; No involvement of IgG autoantibodies against extracellular domains of desmoglein 2 in paraneoplastic pemphigus or inflammatory bowel diseases. J Dermatol Sci 2003, 32: 137
5. Mahoney, M et al; Delineation of diversified desmoglein distribution in stratified squamous epithelia: implications in diseases. Exp Dermatol 2006, 15: 101
6. Nava, P et al; Desmoglein-2: a novel regulator of apoptosis in the intestinal epithelium. MBC 2007, 18: 4565
7. Keim, S et al; Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2. Hybridoma 2008, 27: 249.