Technical datasheet
Description
Desmoglein 3 is a member of the desmosomal cadherin family that comprise the desmogleins and desmocollins. Desmosomes are cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Within the desmosome the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 3 is synthesized in both the basal and the lower suprabasal compartments. The monoclonal antibody 5G11 reacts with the 140 kDa desmoglein 3 protein. Furthermore it is not cross reactive with desmoglein 1 or desmoglein 2.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The monoclonal antibody 5G11 can be used for Western blotting, immuno precipitation and immuno fluoresence. Furthermore the monoclonal antibody 5G11 can be used for immunohistology on paraffin embedded sections. The antibody does not inhibit the biological activity of desmoglein 3.
Use
For immuno fluoresence and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Wahl, J et al; Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 2002, 21: 37
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
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HM2112
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Beta-Catenin, Human, mAb 9F2
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HM2113
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Desmoglein-1, Human, mAb 27B2
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HM2114
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Desmoglein-2, Human, mAb 6D8
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HM2116
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Plakoglobin, Human, mAb 15F11
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HM2118
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Alpha-Catenin, Human, mAb 1G5
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References
1. Wahl, J et al; Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 2002, 21: 37