IgG1, Mouse, biotinylated - HI1002

Innate Immunity

Inflammation

Cell- and tissue damage

Name

IgG1, Mouse, biotinylated

Catalog nr

HI1002 (lot number and expiry date are indicated on the label)

Short description

Mouse IgG1 negative control for the measurement of non-specific binding of mouse monoclonal antibodies of IgG1 isotype.

This control antibody recognizes a rat cell surface marker. Therefore, it cannot be used as a negative control on rat tissues.

The negative control is suitable for use with human, bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.

Size

50 µg

Application

F , FC , P

Technical datasheet

HI1002.pdf

Description

Mouse IgG1 negative control for the measurement of non-specific binding of mouse monoclonal antibodies of IgG1 isotype.

This control antibody recognizes a rat cell surface marker. Therefore, it cannot be used as a negative control on rat tissues.

The negative control is suitable for use with human, bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.

Formulation

0.5 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 0.1% bovine serum albumine and 0.02% sodium azide.

Species

Mouse IgG1

Application

The biotinylated antibody can be used for immunohistochemistry on frozen sections and paraffin embedded sections and flow cytometry.

Use

For immunohistochemistry and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users titrate the antibody for use in their system to a concentration equivalent to the test reagents.

Storage and stability

Product should be stored undiluted at 4°C. Under recommended storage conditions, product is stable for one year.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Also available

HI1001	IgG1, Mouse
HI1003	IgG1, Mouse, FITC
HI1005	IgG2a, Mouse, biotinylated
HI1008	IgG2b, Mouse, biotinylated

MSDS

MSDS purified antibodies with azide.pdf

Protocols

Protocol Immunohistochemistry Frozen.pdf

Protocols

Protocol Flow cytometry.pdf

Protocols

Protocol Immunohistochemistry Paraffin.pdf

Related products		Cat #
IgG2a, Mouse, biotinylated		HI1005
IgG1, Mouse, FITC		HI1003
IgG1, Mouse		HI1001
IgG2b, Mouse, biotinylated		HI1008