IgG2a, Mouse, biotinylated - HI1005

Name

IgG2a, Mouse, biotinylated

Catalog nr

HI1005 (lot number and expiry date are indicated on the label)

Short description

Mouse IgG2a negative control for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a.

The control antibody is raised against activated rat T-helper cells. Therefore, it cannot be used as a negative control in rat tissues.

The negative control is suitable for use with human, bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.

Size

50 µg

Application

Technical datasheet

HI1005.pdf

Description

Mouse IgG2a negative control for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a.

The control antibody is raised against activated rat T-helper cells. Therefore, it cannot be used as a negative control in rat tissues.

The negative control is suitable for use with human, bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.

Formulation

0.5 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 0.1% bovine serum albumine and 0.02% sodium azide.

Species

Mouse IgG2a

Application

The biotinylated antibody can be used for flow cytometry. The antibody is less suitable for intracellular staining.

Use

For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users titrate the antibody for use in their system to a concentration equivalent to the test reagents.

Storage and stability

Product should be stored undiluted at 4°C. Under recommended storage conditions, product is stable for one year.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Also available

HI1004	IgG2a, Mouse
HI1006	IgG2a, Mouse, FITC
HI1002	IgG1, Mouse, biotinylated
HI1008	IgG2b, Mouse, biotinylated

MSDS

MSDS purified antibodies with azide.pdf

Protocols

Protocol Flow cytometry.pdf

Related products		Cat #
IgG2a, Mouse		HI1004
IgG1, Mouse, biotinylated		HI1002
IgG2b, Mouse, biotinylated		HI1008
IgG2a, Mouse, FITC		HI1006