Technical datasheet
Description
CD44, also designated Pgp-1 or H-CAM, is a broadly distributed 85 kD protein. Amongst haematopoietic cells CD44 is expressed on B and T lymphocytes, monocytes and neutrophils. Other CD44-positive cell types include epithelial cells, glial cells, fibroblasts and myocytes. This broad distribution suggest a general role in cell-cell and cell-matrix adhesion. CD44 is involved in T cell activation and adhesion. Triggering of CD44 increases homotypic T cell aggregation mediated via LFA-1. CD44 is linked to the cytoskeleton. Furthermore CD44 has an accessory role in lymphocyte adhesion to high endothelial venules.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The antibody can be used for immunohistology on frozen and paraffin sections. Furthermore the antibody is useful for flow cytometry.
Use
For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Pals, S et al; Identification of a widely distributed 90-kDa glycoprotein that is homologous to the Hermes-1 human lymphocyte homing receptor. J Immunol 1989, 143:851
- Pals, S et al; Expression of lymphocyte homing receptor as a mechanism of dissemination in non-Hodgkin's lymphoma. Blood 1989, 73:885
- Oostendorp, R et al; Antibodies to the beta 1-integrin chain, or ICAM-3 stimulate adhesion of blast colony-forming cells and may inhibit their growth. Exp Hematol 1991, 25:345
- Oostendorp. R et al; Adhesion of human hematopoietic prognitor cells to bone-marrow-derived stromal cells is enhanced by antibodies to CD44. Acta Haematol 1996, 95:243
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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