Name
AIF-1, Human, mAb 1022-5
Catalog nr
HM2184
(lot number and expiry date are indicated on the label)
Short description
Monoclonal antibody 1022-5 reacts with Allograft-inflammatory factor-1 (AIF-1) polypeptide, also known as Ionized calcium-binding adapter molecule 1, Iba-1. AIF-1 is a 17 kDa IFN-gamma inducible Ca 2+ -binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various patholog...
Size
100 µg
Application
IA
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P
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W
Technical datasheet
Description
Monoclonal antibody 1022-5 reacts with Allograft-inflammatory factor-1 (AIF-1) polypeptide, also known as Ionized calcium-binding adapter molecule 1, Iba-1. AIF-1 is a 17 kDa IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis.
Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region.
The monoclonal antibody 1022-5 is cross reactive with rat Allograft-inflammatory factor-1 (rat AIF-1). Human and rat amino acids sequences are 98% homologous.
Cross Reactivity
| Cross reactant | Reactivity |
| Rat | Yes |
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG2b
Application
The monoclonal antibody 1022-5 can be used for Western blotting and immunohistology on paraffin sections. Furthermore the monoclonal antibody 1022-5 is useful as detector in immuno assays.
Use
For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For immunohistology on paraffin sections, a combination of microwave and sodium citrate solution is recommended for antigen retrieval.
Aliases
Ionized calcium-binding adapter molecule 1, Iba-1
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Schluesener, H et al; Allograft-inflammatory factor-1 in rat experimental autoimmune encephalomyelitis, neuritis, and uveitis: expression by activated macrophages and microglial cells. Glia 1998, 24: 244
- Beschorner, R et al; Differential regulation of the monocytic calcium-binding peptides macrophage-inhibiting factor related protein-8 (MRP8/S100A8) and allograft inflammatory factor-1 (AIF-1) following human traumatic brain injury. Acta neuropathol 2000, 100: 627
- Deininger, M et al; The allograft inflammatory factor-1 in Creutzfeldt-Jakob disease brains. Neuropathol Appl Neurobiol 2003, 29: 389
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Also available
| HM2185 | EMAP-II, Human, mAb 546-2 |
| HM2188 | Endostatin, Human, mAb 1837-46 |
| HM2156 | Calprotectin, Human, mAb 27E10 |
| HM2175 | MRP-8, Human, mAb 7C12/4 |
| HM5001 | Nitrotyrosine, mAb HM.11 |
References
- Schluesener, H et al; Allograft-inflammatory factor-1 in rat experimental autoimmune encephalomyelitis, neuritis, and uveitis: expression by activated macrophages and microglial cells. Glia 1998, 24: 244
- Beschorner, R et al; Differential regulation of the monocytic calcium-binding peptides macrophage-inhibiting factor related protein-8 (MRP8/S100A8) and allograft inflammatory factor-1 (AIF-1) following human traumatic brain injury. Acta neuropathol 2000, 100: 627
- Deininger, M et al; The allograft inflammatory factor-1 in Creutzfeldt-Jakob disease brains. Neuropathol Appl Neurobiol 2003, 29: 389
Scientific info
Allograft inflammatory factor-1 as macrophage inflammatory marker
Allograft inflammatory factor-1 (AIF-1) is an evolutionarily conserved, inflammatory protein produced by activated macrophages. AIF-1 is a 147 amino acid calcium-binding protein (~17 kD). AIF-1 is identical to IBA-1 (ionized calcium binding adapter molecule), and is highly homologous to MRF-1 (microglial response factor-1) and Daintain.
Figure: Microglia are normally inconspicuous residents of the central nervous system parenchyma, readily identifiable by their classical rod-shaped nuclei. Their bipolar processes are demonstrated by AIF-1 staining
Allograft inflammatory factor-1 (AIF-1) is an evolutionarily conserved, inflammatory protein produced by activated macrophages. AIF-1 is a 147 amino acid calcium-binding protein (~17 kD). AIF-1 is identical to IBA-1 (ionized calcium binding adapter molecule), and is highly homologous to MRF-1 (microglial response factor-1) and Daintain.
AIF-1 expression is associated with a number of inflammatory conditions among which vascular trauma, arterial injury, skin disorders, atherosclerosis, chronic transplant rejection and brain disorders (for instance Creutzfeldt-Jakob and cerebral infarctions).
In normal human brain, AIF-1 is constitutively expressed by microglial cells. Upon inflammatory, traumatic and ischaemic conditions, AIF-1 is clearly upregulated. AIF-1 can, therefore, be used to study microglial distribution under both normal and pathological conditions.
Figure: Microglia are normally inconspicuous residents of the central nervous system parenchyma, readily identifiable by their classical rod-shaped nuclei. Their bipolar processes are demonstrated by AIF-1 staining
Protocols
Protocols
Protocols
Protocols
| Related products | Cat # | |
|---|---|---|
| Calprotectin, Human, mAb 27E10 | HM2156 | |
| Nitrotyrosine, mAb HM.11 | HM5001 | |
| Endostatin, Human, mAb 1837-46 | HM2188 | |
| EMAP-II, Human, mAb 546-2 | HM2185 | |
| MRP-8, Human, mAb 7C12/4 | HM2175 | |