Technical datasheet
Description
The monoclonal antibody 1G5 reacts with human alpha-catenin. Alpha-catenin belongs together with beta- and gamma-catenin to the catenin family. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. Cadherins are single pass, type I transmembrane proteins that are localized in the adherens junction. To be fully functional, the cadherin cytoplasmic domain must be linked to the actin cytoskeleton through a group of proteins termed catenins: alpha-catenin, beta-catenin and plakoglobin. Alpha catenin interacts with the cadherin indirectly via interactions with beta-catenin or plakoglobin.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The monoclonal antibody 1G5 can be used for Western blotting, immuno precipitation and immunofluorescence.
Use
For Western blotting and immunofluorescence dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Nieman, M et al; Mechanism of extracellular domain-deleted dominant negative cadherins. J Cell Science 1999, 112: 1621
2. Johnson, K et al; P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 1993,
207: 252
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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HM2112
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Desmoglein-1, Human, mAb 27B2
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HM2114
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Desmoglein-2, Human, mAb 6D8
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HM2115
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Desmoglein-3, Human, mAb 5G11
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HM2116
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Plakoglobin, Human, mAb 15F11
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