Name
Beta1 Integrin, Human, mAb BV7
Catalog nr
HM2033
(lot number and expiry date are indicated on the label)
Short description
The monoclonal antibody BV7 recognizes human ß1-integrin. Beta-1 integrin is a ubiquitously expressed ~89 kDa type I transmembrane protein functioning as receptor when heterodimerized with one alpha subunit. It belongs to the integrin beta chain family consisting of four different genes, encoding multiple β-integrins via alternative splicing. Ligand-recognition depends on the composition of the heterodimer: either collagen, fibronectin, VCAM1, laminin...
Size
100 µg
Application
FC
,
FS
,
IA
,
IP
,
W
Technical datasheet
Description
The monoclonal antibody BV7 recognizes human ß1-integrin. Beta-1 integrin is a ubiquitously expressed ~89 kDa type I transmembrane protein functioning as receptor when heterodimerized with one alpha subunit. It belongs to the integrin beta chain family consisting of four different genes, encoding multiple β-integrins via alternative splicing. Ligand-recognition depends on the composition of the heterodimer: either collagen, fibronectin, VCAM1, laminin, cytotactin, osteopontin, epiligrin, thrombospondin and CSPG4 can bind to the integrin-complex. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with α7, β1-integrin regulates cell adhesion and laminin matrix deposition. BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. It is involved in promoting endothelial cell motility and angiogenesis. Furthermore, β1-integrin plays a mechanistic adhesive role during telophase, and is required for the successful completion of cytokinesis.
Upon activation integrins in general, including β1-integrin, are known to exhibit global structural rearrangements and exposure of ligand binding sites. β1-integrin modulation is of importance in tissue repair and regeneration. In cultured primary hippocampal neurons, astrocytes and tissues, cell surface expression of amyloid beta fibrils (key hallmark of Alzheimer’s disease) selectively co-localized with β1-integrin. Preincubation of cells with antibodies against β1-integrin, as well as α1-integrin, greatly enhanced amyloid beta-induced apoptosis, indicating a protective role for integrins in apoptosis.
The monoclonal antibody BV7 does not recognize α5β1 complex and not the cytoplasmic part of the β1-subunit. BV7 binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.
Upon activation integrins in general, including β1-integrin, are known to exhibit global structural rearrangements and exposure of ligand binding sites. β1-integrin modulation is of importance in tissue repair and regeneration. In cultured primary hippocampal neurons, astrocytes and tissues, cell surface expression of amyloid beta fibrils (key hallmark of Alzheimer’s disease) selectively co-localized with β1-integrin. Preincubation of cells with antibodies against β1-integrin, as well as α1-integrin, greatly enhanced amyloid beta-induced apoptosis, indicating a protective role for integrins in apoptosis.
The monoclonal antibody BV7 does not recognize α5β1 complex and not the cytoplasmic part of the β1-subunit. BV7 binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.
Immunogen
Human umbilical vein EC
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
|
|
F |
FC1,3 |
FS1,2,4,5 |
IA |
IF |
IP1 |
P |
W |
|
Yes |
|
● |
● |
● |
|
● |
|
● |
|
No |
|
|
|
|
|
|
|
|
|
N.D. |
● |
|
|
|
● |
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● |
|
N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application FC has been tested by Hycult Biotech. Application IA and W is based on personal communication.
Application notes
FC: Antibody BV7 stains the extracellular domain of beta-1 integrin. HUVEC cells were fixed in 4% paraformaldehyde before staining. Mouse IgG1 isotype was used as negative.
FS: Antibody BV7 functions as an inhibiting antibody. The antibody was functionally tested by adhesion assay (ref 1), chemotaxis (ref 2), patch clamp analysis (ref 3) and neurotoxicity induced apoptosis (ref 4).
Red: isotype control mouse IgG1
Black: irrelevant mouse IgG1 control
Blue: HM2033, clone BV7
Beta-1 integrin expression on HUVEC cells. Flow cytometric detection of human β1-integrin on HUVECs (mAb BV7; Cat# HM2033). Controls and anti-human β1-integrin at 0.4 µg/100000 cells (1 hr 4 °C).
FS: Antibody BV7 functions as an inhibiting antibody. The antibody was functionally tested by adhesion assay (ref 1), chemotaxis (ref 2), patch clamp analysis (ref 3) and neurotoxicity induced apoptosis (ref 4).
Black: irrelevant mouse IgG1 control
Blue: HM2033, clone BV7
Beta-1 integrin expression on HUVEC cells. Flow cytometric detection of human β1-integrin on HUVECs (mAb BV7; Cat# HM2033). Controls and anti-human β1-integrin at 0.4 µg/100000 cells (1 hr 4 °C).
Use
For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of beta1 integrin to be inactivated.
Aliases
CD29, fibronectin receptor subunit beta, integrin VLA-4 subunit beta
Positive control
HT-29 colon carcinoma, Endothelial cells
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Martin-Padura, I et al; A novel mechanism of colon carcinoma cell adhesion to the endothelium triggered by ß1 integrin chain. J Biol Chem 1994, 269: 6124
2. Palmieri, D et al;Trimer carboxyl propeptide of collagen I produced by mature osteoblasts is chemotactic for endothelial cells. J Biol Chem 2000, 275: 32658
3. Martel, V et al; Talin controls exit of the integrin α5β1 from an early compartment of the secretory pathway. J Cell Sci 2000, 113: 1951
4. Hofmann, G et al; HERG K+ channels activation during β1 integrin-mediated adhesion to fibronectin induces an up-regulation of αvβ3 integrin in the preosteoclastic lekemia cell line FLG29.1. J Biol Chem 2001, 276: 4923
5. Bozzo, C et al; Involvement of β1 integrin in βAP-induced apoptosis in human neuroblastoma cells. Mol Cell Neurosci 2004, 25: 1
2. Palmieri, D et al;Trimer carboxyl propeptide of collagen I produced by mature osteoblasts is chemotactic for endothelial cells. J Biol Chem 2000, 275: 32658
3. Martel, V et al; Talin controls exit of the integrin α5β1 from an early compartment of the secretory pathway. J Cell Sci 2000, 113: 1951
4. Hofmann, G et al; HERG K+ channels activation during β1 integrin-mediated adhesion to fibronectin induces an up-regulation of αvβ3 integrin in the preosteoclastic lekemia cell line FLG29.1. J Biol Chem 2001, 276: 4923
5. Bozzo, C et al; Involvement of β1 integrin in βAP-induced apoptosis in human neuroblastoma cells. Mol Cell Neurosci 2004, 25: 1
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Also available
References
1. Martin-Padura, I et al; A novel mechanism of colon carcinoma cell adhesion to the endothelium triggered by ß1 integrin chain. J Biol Chem 1994, 269: 6124
2. Palmieri, D et al;Trimer carboxyl propeptide of collagen I produced by mature osteoblasts is chemotactic for endothelial cells. J Biol Chem 2000, 275: 32658
3. Martel, V et al; Talin controls exit of the integrin α5β1 from an early compartment of the secretory pathway. J Cell Sci 2000, 113: 1951
4. Hofmann, G et al; HERG K+ channels activation during β1 integrin-mediated adhesion to fibronectin induces an up-regulation of αvβ3 integrin in the preosteoclastic lekemia cell line FLG29.1. J Biol Chem 2001, 276: 4923
5. Bozzo, C et al; Involvement of β1 integrin in βAP-induced apoptosis in human neuroblastoma cells. Mol Cell Neurosci 2004, 25: 1
2. Palmieri, D et al;Trimer carboxyl propeptide of collagen I produced by mature osteoblasts is chemotactic for endothelial cells. J Biol Chem 2000, 275: 32658
3. Martel, V et al; Talin controls exit of the integrin α5β1 from an early compartment of the secretory pathway. J Cell Sci 2000, 113: 1951
4. Hofmann, G et al; HERG K+ channels activation during β1 integrin-mediated adhesion to fibronectin induces an up-regulation of αvβ3 integrin in the preosteoclastic lekemia cell line FLG29.1. J Biol Chem 2001, 276: 4923
5. Bozzo, C et al; Involvement of β1 integrin in βAP-induced apoptosis in human neuroblastoma cells. Mol Cell Neurosci 2004, 25: 1
Protocols
Protocols
Protocols
Protocols
Protocols
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|---|---|---|
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