Technical datasheet
Description
The monoclonal HM.2 antibody reacts with the 90 kD glycoprotein Intracellular Adhesion Molecule-1 (ICAM-1). The adhesion molecule ICAM-1 belongs to the immunoglobulin superfamily, C2 subset; it is a ligand for the Integrins LFA-1 and MAC-1 and for CD43.
ICAM-1 is an essential component in many immune-related processes. ICAM-1 links with receptors of the integrin family, thereby mediating cell-cell interactions and allowing for signal transduction. ICAM-1 interacts specifically with its receptors to induce a reversible adhesion interaction. For processes like T cell activation and leucocyte recruitment, normal immune function relies on ICAM-1. Therefore, it is understandable that alterations in ICAM-1 structure or expression are associated with immune disorders. It is important to properly understand the various functions and regulatory mechanisms of ICAM-1, the resulting disease-related failures, and the various treatments.
ICAM-1 is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFalpha) and is expressed by the vascular endothelium, macrophages and lymphocytes
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG1
Application
The monoclonal antibody HM.2 can be used for staining of in vitro cultured cells and the monoclonal antibody HM.2 is useful for for immunohistology on frozen and paraffin sections. Furthermore, the antibody is useful for immuno assays and immuno precipitation.
Use
For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
ICAM-1, CD54
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- van de Stolpe, A et al; Intercellular adhesion molecule-1. J Mol Med 1996; 74:13
- Sligh, J et al; Inflammatory and immune responses are impaired in mice deficiënt in intercellular adehesion molecule 1. PNAS 1993; 90: 8529
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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