Name
Endomucin, Mouse, mAb V.7C7.1
Catalog nr
HM1108 (lot number and expiry date are indicated on the label)
Short description
The monoclonal antibody V.7C7.1 recognizes endomucin, type I membrane protein of 248 amino acids (75 kDa) and shows no signicant homology to any known glycoprotein. As a typical mucin-like glycoprotein, endomucin has a high content of serine and threonine residues, suggesting strong O-glycosylation; the sensitivity to O-sialoglycoprotein endopeptidase indicates that endomucin is also a sialom...
Size
100 µg
Application
F , FC , IP , P , W
Technical datasheet
Description
The monoclonal antibody V.7C7.1 recognizes endomucin, type I membrane protein of 248 amino acids (75 kDa) and shows no signicant homology to any known glycoprotein. As a typical mucin-like glycoprotein, endomucin has a high content of serine and threonine residues, suggesting strong O-glycosylation; the sensitivity to O-sialoglycoprotein endopeptidase indicates that endomucin is also a sialomucin.

Endomucin is an endothelial-specific sialomucin. It is a constitutively expressed endothelial cell surface protein that is found on all venules but is absent from high endothelial venule cells (HEV) of peripheral and mesenteric lymph nodes as well as Peyer's patches, the specialized site for most efficient lymphocyte trafficking. This could indicate an anti-adhesive function of endomucin, as demonstrated for other sialomucins.

Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is another cell adhesion molecule that contains a mucin-like domain and is expressed on HEV in Peyer's patches, mesenteric lymph nodes and on venules in intestinal lamina propria. In the HEV of mesenteric lymph nodes, the mucin-like domain of a subpopulation of MAdCAM-1 molecules contains sulfated carbohydrate side chains that interact with L-selectin.

The presence of three putative protein kinase C phosphorylation sites in the cytoplasmic tail of endomucin indicates that endomucin has the capacity to be a signaling molecule.
Cross Reactivity

Cross reactant

Reactivity (Yes/No)

Human

No

Immunogen
Mouse endomucin-IgG fusion protein
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Rat IgG2a
Application

F

FC

FS

IA

IF

IP

P

W

Yes

No

N.D.

N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot

Use
For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Endomucin-2, mucin-14, gastric cancer antigen Ga34
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
  1. Morgan, S et al; Biochemical characterization and molecular cloning of a novel endothelial-specific sialomucin. Blood 1999, 93: 165
  2. Kuhn, A et al; Expression of endomucin, a novel endothelial sialomucin, in normal and diseased skin. J Invest Dermatol. 2002, 119: 1388
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Also available
References
  1. Morgan, S et al; Biochemical characterization and molecular cloning of a novel endothelial-specific sialomucin. Blood 1999, 93: 165
  2. Kuhn, A et al; Expression of endomucin, a novel endothelial sialomucin, in normal and diseased skin. J Invest Dermatol. 2002, 119: 1388