Name
VAP-1, Mouse, mAb 7-88
Catalog nr
HM1094
(lot number and expiry date are indicated on the label)
Short description
The monoclonal antibody 7-88 recognizes mouse Vascular Adhesion Protein-1 (VAP-1) which is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains a short N-terminal cytoplasmic tail, a single membrane-spanning domain and a large extracellular part. A soluble form of VAP-1 (sVAP-1) has been described, which presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 belongs to enzymes called semicarbamizide-sensitive amine oxidases, which contain copper as a cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia.
Size
100 µg
Application
F
,
FC
,
FS
,
IF
,
IP
Technical datasheet
Description
The monoclonal antibody 7-88 recognizes mouse Vascular Adhesion Protein-1 (VAP-1) which is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains a short N-terminal cytoplasmic tail, a single membrane-spanning domain and a large extracellular part. A soluble form of VAP-1 (sVAP-1) has been described, which presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 belongs to enzymes called semicarbamizide-sensitive amine oxidases, which contain copper as a cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia.
VAP-1 is expressed in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity.
In white adipose tissue of obese and diabetic db-/- mice increased expression of VAP-1 has been observed suggesting that it contributes to the arthrosclerosis and vascular dysfunction observed in these diseases. Moreover, inhibition of VAP-1reduced the accumulation of myeloid cells into tumors and attenuates tumor growth.
The monoclonal antibody 7-88 inhibits migration of granulocytes and monocytes in acute models of inflammation.
VAP-1 is expressed in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity.
In white adipose tissue of obese and diabetic db-/- mice increased expression of VAP-1 has been observed suggesting that it contributes to the arthrosclerosis and vascular dysfunction observed in these diseases. Moreover, inhibition of VAP-1reduced the accumulation of myeloid cells into tumors and attenuates tumor growth.
The monoclonal antibody 7-88 inhibits migration of granulocytes and monocytes in acute models of inflammation.
Immunogen
Vessels from mouse lymph nodes
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin
Species
Rat IgG2b
Application
|
|
F2,3 |
FC2 |
FS2,4 |
IA |
IF5 |
IP1 |
P |
W |
|
Yes |
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● |
● |
|
● |
● |
|
|
|
No |
|
|
|
|
|
|
|
|
|
N.D. |
|
|
|
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Application notes
FC: Antibody 7-88 stains the extracellular domain of mouse VAP-1 in CHO cells transfected with mouse VAP-1 cDNA. . As positive control anti-VAP-1 clone 7-106 was used and as negative control an isotype-matched control antibody. (Ref. 2)
IHC-F: Tissue sections were fixed in acetone and incubated with antibody 7-88 for 20 minutes at room temperature. . As negative control an irrelevant isotype-matched antibody was used (Ref.2).
FS: Antibody 7-88 (200µg) was intravenously injected which resulted in the inhibition of leukocyte trafficking in inflamed peritoneum. (Ref.2).
VAP-1 expression in mouse muscle tissue; frozen sections.
IHC-F: Tissue sections were fixed in acetone and incubated with antibody 7-88 for 20 minutes at room temperature. . As negative control an irrelevant isotype-matched antibody was used (Ref.2).
FS: Antibody 7-88 (200µg) was intravenously injected which resulted in the inhibition of leukocyte trafficking in inflamed peritoneum. (Ref.2).
VAP-1 expression in mouse muscle tissue; frozen sections.
Use
For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user’s experimental setting.
Aliases
Amine oxidase copper containing 3; AOC3; SSAO; membrane copper amine oxidase
Positive control
Mouse VAP-1-transfected CHO cells (ref. 2)
Negative control
Mock-transfected CHO cells (Ref. 2)
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
References
1. Abella, A et al; Adipocytes release a soluble form of VAP-1/SSAO by a metalloprotease-dependent process and in a regulated manner. Diabetologia 2004, 47: 429
2. Merinen, M et al; Vascular adhesion protein-1 is involved in both acute and chronic inflammation in the mouse. Am J Path 2005, 166: 793
3. Bonder, C et al; Rules of recruitment for Th1 and Th2 lymphocytes in inflamed liver: a role for alpha-4 integrin and vascular adhesion protein-1. Immunity 2005, 23: 153
4. Marttila-Ichihara, F et al; Small-molecule inhibitors of vascular adhesion protein-1 reduce the accumulation of myeloid cells into tumors and attenuate tumor growth in mice. J Immunol 2010, 184: 3164
5. Iffiú-Soltész, Z et al; Increased primary amine oxidase expression and activity in white adipose tissue of obese and diabetic db-/- mice. J Neural Transm 2011, DOI: 10.1007/s00702-011-0586-9
2. Merinen, M et al; Vascular adhesion protein-1 is involved in both acute and chronic inflammation in the mouse. Am J Path 2005, 166: 793
3. Bonder, C et al; Rules of recruitment for Th1 and Th2 lymphocytes in inflamed liver: a role for alpha-4 integrin and vascular adhesion protein-1. Immunity 2005, 23: 153
4. Marttila-Ichihara, F et al; Small-molecule inhibitors of vascular adhesion protein-1 reduce the accumulation of myeloid cells into tumors and attenuate tumor growth in mice. J Immunol 2010, 184: 3164
5. Iffiú-Soltész, Z et al; Increased primary amine oxidase expression and activity in white adipose tissue of obese and diabetic db-/- mice. J Neural Transm 2011, DOI: 10.1007/s00702-011-0586-9
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Also available
| HM1013 | PECAM-1, Mouse, mAb MEC7.46 |
| HM1015 | CD34, Mouse, mAb MEC14.7 |
| HM1050 | JAM-A, Mouse, mAb BV12 |
| HM1084 | PECAM-1, Mouse, mAb ER-MP12 |
| HM2213 | VAP-1, Human, mAb 174-5 |
References
1. Abella, A et al; Adipocytes release a soluble form of VAP-1/SSAO by a metalloprotease-dependent process and in a regulated manner. Diabetologia 2004, 47: 429
2. Merinen, M et al; Vascular adhesion protein-1 is involved in both acute and chronic inflammation in the mouse. Am J Path 2005, 166: 793
3. Bonder, C et al; Rules of recruitment for Th1 and Th2 lymphocytes in inflamed liver: a role for alpha-4 integrin and vascular adhesion protein-1. Immunity 2005, 23: 153
4. Marttila-Ichihara, F et al; Small-molecule inhibitors of vascular adhesion protein-1 reduce the accumulation of myeloid cells into tumors and attenuate tumor growth in mice. J Immunol 2010, 184: 3164
5. Iffiú-Soltész, Z et al; Increased primary amine oxidase expression and activity in white adipose tissue of obese and diabetic db-/- mice. J Neural Transm 2011, DOI: 10.1007/s00702-011-0586-9
2. Merinen, M et al; Vascular adhesion protein-1 is involved in both acute and chronic inflammation in the mouse. Am J Path 2005, 166: 793
3. Bonder, C et al; Rules of recruitment for Th1 and Th2 lymphocytes in inflamed liver: a role for alpha-4 integrin and vascular adhesion protein-1. Immunity 2005, 23: 153
4. Marttila-Ichihara, F et al; Small-molecule inhibitors of vascular adhesion protein-1 reduce the accumulation of myeloid cells into tumors and attenuate tumor growth in mice. J Immunol 2010, 184: 3164
5. Iffiú-Soltész, Z et al; Increased primary amine oxidase expression and activity in white adipose tissue of obese and diabetic db-/- mice. J Neural Transm 2011, DOI: 10.1007/s00702-011-0586-9
Protocols
Protocols
Protocols
| Related products | Cat # | |
|---|---|---|
| PECAM-1, Mouse, mAb ER-MP12 | HM1084 | |
| JAM-A, Mouse, mAb BV12 | HM1050 | |
| CD34, Mouse, mAb MEC14.7 | HM1015 | |
| PECAM-1, Mouse, mAb MEC7.46 | HM1013 | |
| VAP-1, Human, mAb 174-5 | HM2213 | |