Technical datasheet
Description
Mouse IgG
2a negative control for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG
2a.
The control antibody is raised against activated rat T-helper cells. Therefore, it cannot be used as a negative control in rat tissues.
The negative control is suitable for use with human, bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
Formulation
0.5 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumine and 0.02% sodium azide.
Species
Mouse IgG2a
Application
The antibody can be used as negative control for flow cytometry. The antibody is less suitable for intracellular staining.
Use
For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users titrate the antibody for use in their system to a concentration equivalent to the test reagents.
Storage and stability
Product should be stored undiluted at 4°C or at -20°C if preferred. Avoid repeated freezing and thawing as this may denature the antibody. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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