Technical datasheet
Description
The polyclonal antibody reacts with human Hakai, a c-Cbl-like protein. Hakai is an E-cadherin binding protein, which has been identified as an E3 ubiquitin-ligase. Hakai contains SH2, RING, zinc-finger and proline-rich domains. Hakai interacts with E-cadherin in a tyrosine phosphorylation-dependent manner, inducing ubiquitination of the E-cadherin complex. Expression of Hakai in epithelial cells disrupts cell-cell contacts and enhances endocytosis of E-cadherin and cell motility. By promoting the endocytosis and dynamic recycling or destruction of E-cadherin complexes, Hakai is able to control epithelial-mesenchymal transitions under physiological and pathological conditions.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Rabbit IgG
Application
The polyclonal antibody to human Hakai can be used for Western blot, immunofluorescence and immunohistology on frozen sections.
Use
For Western blotting, immunofluorescence and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Fujita, Y et al; Hakai, a c-Cbl-like protein, ubiquitinates and induces endocytosis of the E-cadherin complex. Nat Cell Biol 2002, 4: 222
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
References
1. Fujita, Y et al; Hakai, a c-Cbl-like protein, ubiquitinates and induces endocytosis of the E-cadherin complex. Nat Cell Biol 2002, 4: 222