Technical datasheet
Description
The Horseradish Peroxidase (HRP)-conjugated polyclonal antibody to mouse IgG + IgM reacts with the heavy chains on mouse IgG and IgM and with light chains common to most mouse immunoglobulins. The affinity-purified antibody has been isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose gels. Due to a special purification step it shows minimal cross-reaction to human, bovine and horse serum proteins. The polyclonal antibody to mouse IgG + IgM does not react with non-immunoglobulin serum proteins. The antibody has been tested by Elisa to ensure the highest possible quality.
Formulation
1.2 mg lyophilized affinity-purified antibody in PBS containing stabilizing protein. Reconstitute the vial by injection of 1.5 ml distilled or de-ionized water (Caution: vial is under vacuum).
Species
Goat IgG
Application
The HRP-conjugated polyclonal antibody to mouse IgG + IgM is useful for immuno assays as detection antibody, immunohistology on frozen and paraffin sections and Western blotting.
Use
For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. For most applications we suggest dilution ranges from 1:500 - 1:5000. Warning: do not add sodium azide since this will substantially inhibit the enzyme activity of horseradish peroxidase.
Storage and stability
Lyophilized product should be stored at 4°C. Store stock solution in aliquots at -70°C. Alternatively the product can be stored at -20°C if an equal volume of glycerol is added to the solution for a final concentration of 50%. Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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