Technical datasheet
Description
A rat IgG
1 negative control for the measurement of non-specific binding of rat monoclonal antibodies of isotype IgG
1.
This antibody recognizes a human cell surface marker. Therefore, it cannot be used as a negative control in human tissues.
The negative control is suitable for use with mouse tissues.
Formulation
0.5 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumine and 0.02% sodium azide.
Species
Rat IgG1
Application
The antibody can be used for immuno assay, immunohistochemistry on frozen sections and flow cytometry. Furthermore, the antibody is useful as an ELISA standard.
Use
For immuno assay, immunohistochemistry and flow cytometry dilutions to be used depend on detection system applied. It is recommended that the user titrates the antibody for use in their system to a concentration equivalent to that of their test reagents.
Storage and stability
Product should be stored undiluted at 4°C or at -20°C if preferred. Avoid repeated freezing and thawing as this may denature the antibody. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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