Technical datasheet
Description
The monoclonal antibody recognizes leucine-rich alpha-2-glycoprotein (LRG), a member of the leucine-rich repeat family. LRG is a plasma protein of approximately 50 kDa. LRG consists of 312 amino acids. It has 1 galactosamine and 4 glucosamine oliogosacharides attached and has 2 intrachain disulfide bonds. Leucine comprises 66 of the 312 amino acids, and LRG contains at least eight 24-amino acid leucine-rich repeats. The consensus of these residues, termed leucine-rich repeats (LRR), has been identified as an LRR-containing domain. The primary function of LRRs is to provide versatile structural framework of the formation of protein-protein interactions. LRG is expressed during granulocyte differentiation.
Although several family members of the leucine-rich repeat family have been shown to be involved in signal transduction, protein-protein interaction, or cell adhesion and development, a function for LRG remains elusive. LRG has been reported as a biomarker for certain diseases including microbial infections and cancer. LRG can be detected in human neutrophils and progenitor cells, but not in peripheral blood mononuclear cells.
The monoclonal antibody 2F5.A2 can be used for immuno purification of human LRG.
Immunogen
Human LRG, covalently coupled to hemocyanin using glutaraldehyde
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG1
Application
| | F | FC | FS | IA1,2 | IF | IP | P | W | | Yes | | | | ● | | | | | | No | | | | | | | | | | N.D. | ● | ● | ● | | ● | ● | ● | ● | |
N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot. Application IA has been tested by Hycult Biotech. |
Application notes
IA: Antibody was used as detector for LRG on cytochrome C coated plates. Concentration antibody used was 1 µg/ml.
Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Leucine-rich alpha-2-glycoprotein, LRG
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Weivoda, S et al; ELISA for human serum leucine-rich alpha-2-glycoprotein-1 employing cytochrome c as the capturing ligand. J Immunol Meth 2008, 336: 22
- Codina, R et al; Cytochrome c-induced lymphocyte death from the outside in: inhibition by serum leucine-rich alpha-2-glycoprotein-1. Apoptosis 2010, 15: 139
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Also available
|
HM2100
|
SAA-1, Human, mAb Reu86.1
|
|
HM2101
|
SAA-1, Human, mAb Reu86.5
|
|
HM2241
|
PTX3, Human, mAb MNB1
|
|
HM2242
|
PTX3, Human, mAb MNB4
|
|
HM2061
|
MBL, Human, mAb 3E7
|