Technical datasheet
Description
The monoclonal antibody 6B1 recognizes human triggering receptor expressed on myeloid cells 1 (TREM-1). TREM-1 is a transmembrane glycoprotein belonging to a family related to the natural killer cell receptors. TREM-1 is an important activating receptor, of 26 kDa, involved in the innate inflammatory response and in sepsis. TREM-1 is expressed at low levels in the early development of the hematopoietic system and in the promonoistic stage and at high levels on neutrophil granulocytes (PMN), monocytes, and macrophage subsets. The expression of TREM-1 is upregulated by microbial products, that is, by toll-like receptor ligands such as lipoteichoic acid (LTA) of Gram-positive or lipopolysaccharide (LPS) of Gram-negative bacteria. Ligation of TREM-1 is synergistic with TLR agonists on the activation of receptor bearing cells. Platelets express a natural ligand for TREM-1. Receptor ligation activates the full repertoire of effector functions.
TREM-1 is also produced in a soluble form (sTREM-1) of 17 kDa which is released in humans after endotoxin exposition or in patients suffering from severe pneumonia or sepsis. sTREM-1 can be measured in biological fluids and may be useful as a diagnostic tool. The monoclonal antibody 6B1 recognizes both membrane bound and soluble TREM-1.
Also, the monoclonal antibody 6B1 neutralizes the interaction between TREM-1 and its ligand.
Immunogen
Recombinant TREM-1 - IgG1 fusion protein.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
| | F | FC | FS | IA | IF | IP | P | W | | Yes | | ● | ● | ● | | | | ● | | No | | | | | | | | | | N.D. | ● | | | | ● | ● | ● | | |
N.D.=Not Determined; F=Frozen sections; FC=Flow cytometry; FS=Functional studies; IA=Immuno assays; IF=Immuno fluorescence; IP=Immuno precipitation; P=Paraffin sections; W=Western blot |
Use
For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Aliases
TREM-1, triggering receptor expressed on myeloid cells 1, TREM1
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Radsak, M et al; Soluble triggering receptor expressed on myeloid cells 1 is released in patients with stable chronic obstructive pulmonary disease. Clin Dev Immunol 2007, 2007: 52040
- Haselmayer, P et al; TREM-1 ligand expression on platelets enhances neutrophil activation. Blood 2007, 110: 1029
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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