Technical datasheet
Description
The monoclonal antibody 43 reacts with the hydrophilic backbone of free lipid A. This antibody only reacts with lipid A not covalent bound to LPS. LPS is chemically composed of a lipid part, lipid A, which is responsible for the endotoxic activities of LPS, and a heteropolysaccharide which generally consists of an oligosaccharide, termed the core region, and, in some bacterial families, of the so-called O chain, composed of repeating oligosaccharide units.
Formulation
1 ml (> 200 µg/ml) culture medium with a low endotoxin level containing 0.02% sodium azide.
Species
Mouse IgM
Application
The antibody can be used for immunoassays and Western blotting.
Use
For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at -20°C. Under recommended storage conditions, product is stable for one year.
References
1. Kuhn, H et al; Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A. Infect Immun 1992, 60: 2201 2. Kuhn, H et al; The antibody reactivity of monoclonal lipid A antibodies in influenced by the acylation pattern of lipid A and the assay system employed. Immunobiol 1993, 189: 457
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
References
1. Kuhn, H et al; Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A. Infect Immun 1992, 60: 2201
2. Kuhn, H et al; The antibody reactivity of monoclonal lipid A antibodies in influenced by the acylation pattern of lipid A and the assay system employed. Immunobiol 1993, 189: 457