Technical datasheet
Description
Monoclonal antibody 1837-46 reacts with endostatin, a 20 kDa C-terminal fragment of collagen XVIII. Endostatin is able to evoke non-uniform response for proliferation, cell mount and migration of entothelial cells, with different endostatin binding characteristic, leads to the assumption that endostatin effect is strongly dependent from endothelial cell type. Furthermore endostatin inhibits angiogenesis and tumor growth in vivo by inducing apoptosis in endothelial cells. The local delivery of endostatin seems to specifically affect tumor-associated microvessels by reduction of the vessel density, diameter and functionality. Tumor cell migration and invasion was greatly reduced in the endostatin treated animals. Endostatin is non-toxic and does not induce acquired drug resistance and has therefore become a potent new therapy strategy in solid neoplasias. This therapy appear to have high potential not only for the treatment of gliomas, the most common brain tumours, but also of other tumours. The ability of endostatin to inhibit neoangiogenesis is mediated, at least in part, by Zn2+ binding and elastase processing. Widespread endostatin expression was found in elastic fibers in vessel walls and in some other basement membrane zones. Endostatin is released by neurons to accumulate in amyloid plaques in Alzheimer's disease.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG2b
Application
The monoclonal antibody 1837-46 can be used for Western blotting, flow cytometry and immunohistology on both frozen and paraffin sections. Furthermore the monoclonal antibody 1837-46 is useful as detector in immuno assays.
Use
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For staining of paraffin sections antigen retrieval (microwave irradiation (2x7 minutes) in citrate buffer) is advised.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Deininger, M et al; Accumulation of endostatin/collagenXVIII in brains of patients who died with cerebral malaria. J Neuroimmunol 2002, 131: 216
- Deininger, M et al; Aberrant neuronal and paracellular deposition of endostatin in brains of patients with alzheimer's disease. J Neurosci 2002, 22: 10621
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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