Technical datasheet
Description
The monoclonal antibody LHC7.15 recognizes RNA-binding protein FUS also known as fusion (involved in t(12;16) in malignant liposarcoma) (TLS) and hnRPN P2. The antigen is differentially distributed between the nucleus and the cytoplasm in live and apoptotic cells. In late apoptotic cells, the antigen coalesces into aggregates that bleb from the cell surface. FUS is a multifunctional protein of 69 kD and binds DNA, RNA, and several known ribonucleoprotein autoantigens. The monoclonal antibody LHC7.15 binds nuclei in a speckled pattern reminiscent of the binding of anti-spliceosomal autoantibodies, and to sites in the cytoplasm of live cells. Upon induction of apoptosis, LHC7.15 binds intensely to nuclei before nuclear fragmentation, but is unreactive against nuclear fragments. In later stages of apoptosis, LHC7.15 binds large cytoplasmic granules that form near the plasma membrane, occupy a subset of surface blebs, and separate as apoptotic bodies from the remainder of the apoptotic cells.
Immunogen
Spontaneous autoantibody
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG2a
Application
| | F | FC | FS | IA | IF | IP | P | W | | Yes | | | | | ● | ● | | ● | | No | | | | | | | | | | N.D. | ● | ● | ● | ● | | | ● | | |
N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot |
Use
For immuno fluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
TLS, FUS1, hnRNP-P2, fusion derived from t(12;16) malignant liposarcoma
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Radic, M et al; Heterogeneous nuclear ribonucleoprotein P2 is an autoantibody target in mice deficient for Mer, Axl, and Tyro3 receptor tyrosine kinases. J Immunol 2006, 176: 68
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.