Technical datasheet
Description
The monoclonal antibody M2139 reacts with the J1 epitope (triple helical position 551-564) of collagen type II. Collagen is a structural protein in bone, cartillage and connective tissue. Collagen type II (CII) is the major collagen of the nucleus pulposa (a component of spine), cartilage and vitreous (a component of the eye). The most commonly used animal model for rheumatoid arthritis (RA) is the collagen-induced arthritis (CIA). Transfer of collagen type II specific monoclonal antibodies induces an acute form of arthritis (collagen type II antibody induced arthritis, CAIA). The monocolonal antibody M2139 has been shown to induce CAIA in naïve mice after injection of lipopolysacharide (LPS). However, in combination with the monoclonal antibody CIIC1, binding to the C1 epitope of CII, the pair of monoclonal antibodies induce arthritis in different strains of mice without any other stimulants. The presence of secondary stimulus, LPS, increases the disease incidence and severity. The monoclonal antibody M2139 is cross reactive with rat, bovine, chicken and human CII.
Cross Reactivity
| Cross reactant |
Reactivity (Yes/No) |
| Rat |
Yes |
| Bovine |
Yes |
| Chicken |
Yes |
| Human CII |
Yes |
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS and 0.1% bovine serum albumin
Species
Mouse IgG2b
Application
The monoclonal antibody M2139 can be used for functional studies and for immunohistology on frozen and paraffin embedded sections. Furthermore the monoclonal antibody M2139 is useful in immunoassays as coating and detection.
Use
For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Nandakumar, K et al; Collagen Type II-specific monoclonal antibody-induced arthritis in mice. Description of the disease and the influence of age, sex and genes. Am J Pathol 2003, 163: 1827
- Nandakumar, K et al; Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells. Arthritis Res Ther 2004, 6: R544
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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