Technical datasheet
Description
The polyclonal antibody recognizes mouse Spi-6, also known as serpin B9, a cytoplasmic serine protease inhibitor of ~42 kD. Spi-6 is expressed in dendritic cells, lymphocytes and specific cell types at immune-privileged sites. It inactivates granzyme B, a natural occurring inducer of programmed cell death. As such, Spi-6 functions as anti-apoptotic protein. In cytotxic T cells (CTL), Spi-6 is required to protect clonal bursts (expansion) from granzyme B induced cell death. However, it is not required for the development of memory cells. Expression of SPI-6 is of crucial importance in the escape of several tumors from a CTL response and is, therefore, a parameter that influences the feasibility of CTL-mediated immunotherapy of cancer. The polyclonal antibody shows no crossreactivity with mouse proteins related to Spi-6.
Immunogen
Recombinant GST-Spi-6
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Rabbit Ig
Application
The polyclonal antibody can be used for flow cytometry. Furthermore, the polyclonal antibody is useful for Western blotting.
Use
For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Cytoplasmic antiproteinase 3, serpin B9, PI-9
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Zhang, M et al; Differential survival of cytotoxic T cells and memory cell precursors. J Immunol 2007, 178: 3483
- Zhang, M et al; Serine protease inhibitor 6-deficient mice have increased neutrophil immunity to Pseudomonas aeruginosa. J Immunol 2007, 179: 4390
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
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