Technical datasheet
Description
Monoclonal antibody UA009 reacts with rat CD36 (85-kDa), a cell surface class B scavenger receptor. Recently, CD36 has been identified as a protein that is required for TLR2 recognition of di-acylated bacterial lipopeptides and lipoteichoic acid2. CD36 is a heavily N-glycosylated transmembrane protein with two short intracellular domains and a large extracellular domain. CD36 expression is primarily controlled by the transcription heterodimer PPARg-RXR (peroxisome proliferator-activated receptor-g-retinoid-X-receptor). CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signalling and adaptor molecules. CD36 is expressed on monocytes, macrophages, endothelial cells, hepatocytes, erythrocytes, adipocytes, cardiomyocytes and red skeletal muscle. Importantly it is expressed by steriodogenic cells in the adrenal gland, testis and ovary. CD36 has been implicated in many biological processes including angiogenesis, phagocytosis, inflammation, and lipid and glucose metabolism. CD36 is associated with Src-family kinases and with the integrins alpha3beta1 and alpha6beta1. It is a receptor and transporter of oxidized lipids and long chain fatty acids. CD36 has been shown to function as phagocytic receptor for apoptotic cells. Several in vivo models support the role of the thrombospondin / CD36 system in angiogenesis and tumor growth. An important role for CD36 has been found in Malaria as major receptor for P. falciparum-infected red blood cells. Many different ligands have been reported to interact with CD36, suggesting that CD36 could recognize a structure-based domain rather than specific contact residues. Monoclonal antibody UA009 blocks the biological activity of CD36. It inhibits the uptake of apoptotic thymocytes by macrophages.
Formulation
0.5 mg (100 µg/ml) 0.2 µm filtered antibody solution in PBS (exact concentration is indicated on the label).
Species
Mouse IgG1
Application
The monoclonal antibody UA009 can be used for immuno assays, immuno precipitation, flow cytometry and immunohistology on frozen sections. Furthermore the monoclonal antibody UA009 is useful for neutralization of the biological activity of CD36.
Use
For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of CD36 to be inactivated.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Zhang, X et al; CD36/fatty acid translocase in rats: distribution, isolation from hepatocytes, and comparison with scavenger receptor SR-BI. Lab Invest 2003, 83: 317
- Beutler, B et al; Inferences, questions and possibilities in Toll-like receptor signalling. Nature 2004, 430: 257
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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