Technical datasheet
Description
The monoclonal antibody ECE.2 recognizes mouse monocyte chemoattractant protein 1 (MCP-1). The murine JE gene encodes the monocyte-specific cytokine monocyte chemotactic protein 1 (MCP-1). MCP-1 is a CC chemokine of 76 amino acids (~11 kDa) and is chemotactic for monocytes and basophils but not neutrophils and eosinophils. MCP-1 is expressed by smooth muscle cells (SMC), macrophages, endothelial cells, keratinocytes and fibroblasts in response to inflammatory stimuli such as interleukin 1β and tumor necrosis factor α. MCP-1 has been implicated in a variety of inflammatory processes, including inflammatory bowel disease, rheumatoid arthritis, asthma, nephritis, and parasitic and viral infections. MCP-1 antigen is not detected in the endothelium or SMC of normal arteries. MCP-1 has also been shown to exhibit biological activities other than chemotaxis. It can induce the proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer)
MCP-1 signals via the CCR2 receptor, and is critical for aneurysm formation because of its stability to recruit leukocytes. These leukocytes produce extracellular matrix-degrading MMPs, thereby inductin aortic remodelling and dilatation. Interleukin-6 is also involved in this amplification loop accelerating vascular inflammation. MCP-/- mice display significantly delayed wound re-epithelialization, and also delayed wound angiogenesis.
Cross Reactivity
|
Cross reactant
|
Reactivity
|
|
Human
|
No
|
Immunogen
Synthetic peptide corresponding to residues 102-130 of mouse MCP-1
Formulation
0.5 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Rat IgG1
Application
|
|
F3
|
FC
|
FS
|
IA
|
IF
|
IP
|
P2
|
W1
|
|
Yes
|
●
|
|
|
|
|
|
●
|
●
|
|
No
|
|
|
|
|
|
|
|
|
|
N.D.
|
|
●
|
●
|
●
|
●
|
●
|
|
|
N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application notes
F: Sections (6 µm) were fixed with 4% PFA, blocked using 0.1% Triton-X and 5% serum for 15min at 37°C or o/n 4 °C and then incubated with 2 µg/ml antibody for 2h at 37°C.
P: Fixation in 10% buffered formalin; 5 µm sections
W: Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk. The blot was incubated with antibody (0.8 µg/ml) for 20min at RT.
Use
For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Aliases
C-C motif chemokine 2, Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2, Sigje
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Zoja, C et al; Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol 1997, 8: 720
2. Kimura, Y et al; Facilitating action of asiaticoside at low doses on burn wound repair and its mechanism. Eur J Pharmacol 2008, 584: 415
3. Tieu, B et al. An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice. J Clin Invest 2009, 119: 3637
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
References
1. Zoja, C et al; Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol 1997, 8: 720
2. Kimura, Y et al; Facilitating action of asiaticoside at low doses on burn wound repair and its mechanism. Eur J Pharmacol 2008, 584: 415
3. Tieu, B et al. An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice. J Clin Invest 2009, 119: 3637