Technical datasheet
Description
The antibody is specific for natural and recombinant mouse GM-CSF. It inhibits the induction of the proliferation of BCL
1 cells by GM-CSF.
GM-CSF is a 23 kDa glycoprotein with diverse effects on immune and non-immune cells. It induces differentiation of granulocyte, macrophage and eosinophil precursor cells. Proliferation of monocyte-macrophages, T lymphocytes, keratinocytes and endothelial cells is stimulated by GM-CSF. In addition, GM-CSF alters the functional properties of mature granulocytes, macrophages, eosinophils and basophils.
GM-CSF is produced by T lymphocytes, macrophages and several cell types in extreme dullary sites, where it may act in a paracrine manner to regulate the local response to antigenic challenge.
Formulation
Lyophilized product in PBS, containing 500 µg. Reconstitute the vial by injection of 0.5 ml sterile distilled or deionised water (Caution: vial is under vacuum).
Species
Rat IgG2a
Application
The antibody can be used for inhibition of biological activity of GM-CSF in functional studies and for immuno assay. Furthermore the antibody is useful for Western blotting, immunoprecipitation, staining of frozen sections and for intracellular staining of permeabilized cells.
Use
For Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralisation of biological activity in vitro dilutions have to be made according to the amount of GM-CSF to be inactivated.
Storage and stability
Lyophilized product should be stored at 4°C. Store stock solution in aliquots at -20°C. Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.
References
1. O'Garra, A et al; The BCL1 B lymphoma responds to IL-4, IL-5, and GM-CSF. Cell Immunol 1989, 123: 189 2. Ruef, C et al; Granulocyte-macrphage colony-stimulating factor: pleiotropic cytokine with potential clinical usefulness. Rev Infect Dis 1990, 12: 41
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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