Technical datasheet
Description
The monoclonal antibody MaP.VIP recognizes mouse Viperin, a 42 kDa protein belonging to the RSAD2 family. Viperin is the abbreviation of Virus inhibitory protein endoplasmic reticulum-associated interferon-inducible. It is an evolutionary conserved protein that is highly inducible by both type I and type II interferons. However, little or no induction by interferon gamma is observed in monocytic cell lines. Infection by many viruses, including human cytomegalovirus (hCMV), hepatitis C virus, yellow fever virus and Sendai virus, strongly induces viperin expression, suggesting a role in the host antiviral response. Viperin has also been shown to be important for the host anti-HIV responses. Furthermore, expression of viperin inhibits influenza replication by perturbing its release from the plasma membrane. Viperin expression alters plasma membrane fluidity by affecting formation of lipid rafts which are detergent-resistant membrane microdomains known to be the sites of influenza virus budding. HCMV infection induces the redistribution of viperin from the endoplasmic reticulum to the Golgi complex and ultimately to cytoplasmic vacuoles, suggesting that viperin may function at a distinct level in the viral lifecycle, at the point of glycosylated viral protein transport.
Cross Reactivity
| Cross reactant |
Reactivity (Yes/No) |
| Human Viperin |
Yes |
Immunogen
Recombinant mouse viperin fragment (residues 92-362)
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG2a
Application
| | F | FC | FS | IA | IF | IP | P | W | | Yes | | ● | | | ● | ● | | ● | | No | | | ● | | | | | | | N.D. | ● | | | ● | | | ● | | |
N.D.=Not Determined; F=Frozen sections; FC=Flow cytometry; FS=Functional studies; IA=Immuno assays; IF=Immuno fluorescence; IP=Immuno precipitation; P=Paraffin sections; W=Western blot |
Use
For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Radical S-adenosine methionine domain-containing protein 2, Virus inhibitory protein endoplasmic reticulum-associated interferon-inducible, Cytomegalovirus-induced gene 5 protein, Cig5
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Wang, X et al; The interferon-inducible protein viperin inhibits influenza virus release by perturbing lipid rafts. Cell Host Microbe 2007, 2: 96
- Lackman, R et al; Innate immune recognition triggers secretion of lysosomal enzymes by macrophages. Traffic 2007, 8: 1179
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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