Technical datasheet
Description
The antibody reacts with human monocyte chemotactic protein-1 (MCP-1) both natural and recombinant. MCP-1 is a member of the CC subfamily of chemokines. MCP-1 is a pro-inflammatory cytokine which acts both as an activation factor for monocytes and as chemokine for monocytic cells. The antibody inhibits the biological activity of natural and recombinant human MCP-1. The antigen specificity was assessed by ELISA. The antigen specificity was further assessed by immuno precipitation and Western blot. No cross-reactivities with other cytokines have been detected.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Rabbit IgG
Application
The antibody can be used for immuno assays, immuno precipitation and neutralization of human MCP-1 in bio-assays. Furthermore the antibody can be used for Western blotting and to stain MCP-1 containing cells in frozen sections as well as in cells cultured on cover slips.
Use
For Western blotting and immuno histology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amount of MCP-1 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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