Name
IL-8, Human, ELISA kit
Catalog nr
HK310
(lot number and expiry date are indicated on the label)
Short description
Interleukin-8 (IL-8) belongs to the CXC chemokine family. This cytokine has a wide range of proinflammatory properties ranging from its main function as chemoattractant for neutrophils, basophils and a subpopulation of lymphocytes, to activation of neutrophils. This ELR motive (Glu-Leu-Arg motive in NH2 terminus) positive CXC chemokine is a potent activator of angiogenesis. In vivo , IL-8 is...
Size
2 x 96 det.
Application
Manual
Description
Interleukin-8 (IL-8) belongs to the CXC chemokine family. This cytokine has a wide range of proinflammatory properties ranging from its main function as chemoattractant for neutrophils, basophils and a subpopulation of lymphocytes, to activation of neutrophils. This ELR motive (Glu-Leu-Arg motive in NH2 terminus) positive CXC chemokine is a potent activator of angiogenesis. In vivo, IL-8 is detected in conditions of inflammation. High levels of IL-8 are observed during septic episodes in seriously ill patients. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis. In vitro, multiple cell types can produce IL-8. The cytokine can be rapidly detected after activation of cells with a wide variety of activators; endotoxin is a potent inducer of IL-8 release by monocytes and endothelial cells. The human IL-8 ELISA kit shows cross reactivity with most old world monkeys.
Cross Reactivity
| Cross reactant |
Reactivity (Yes/No) |
| most old world monkeys | Yes |
Application
The Human IL-8 ELISA has been developed for the quantitative measurement of natural and recombinant human IL-8 in serum, plasma, and cell culture medium. In serum or plasma samples human IL-8 can be measured accurately if serum or plasma samples are diluted at least 2 times. Most reliable results are obtained if EDTA plasma is used.
Features
- Minimum concentration which can be measured is 5 pg/ml human IL-8.
- Measurable concentration range of 4-1,000 pg/ml.
- Working volume of 100 µl/well.
Typical standard curve
Principle
- The human IL-8 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4½ hours.
- The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay.
- Samples and standards are captured by a solid bound specific antibody.
- Biotinylated tracer antibody will bind to captured human IL-8.
- Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody.
- Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB).
- The enzyme reaction is stopped by the addition of citric acid.
- The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human IL-8 standards (log).
- The human IL-8 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least six months. After reconstitution the reagents are stable for 1 month if stored at 2-8°C.
References
- Turner, P et al; Role of kinins in seasonal allergic rhinitis: icatibant, a bradykinin B2 receptor antagonist, abolishes the hyperresponsiveness and nasal eosinophilia induced by antigen. J Allergy Clin Immunol 2001, 107: 105
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
References
- Turner, P et al; Role of kinins in seasonal allergic rhinitis: icatibant, a bradykinin B2 receptor antagonist, abolishes the hyperresponsiveness and nasal eosinophilia induced by antigen. J Allergy Clin Immunol 2001, 107: 105
MSDS
| Related products | Cat # | |
|---|---|---|
| IP-10, Human, ELISA kit | HK311 | |
| MCP-1, Human, ELISA kit | HK306 | |
| TNF-alpha, Human, ELISA kit | HK307 | |