Technical datasheet
Description
Plasminogen activator inhibitor type-1 (PAI-1), a member of the serine protease inhibitor (serpin) superfamily is an important protein in the regulation of fibrinolysis. PAI-1 is unique among the serpins because of its functional and conformational flexibility. PAI-1 is the most important physiological inhibitor of both tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA). Increased PAI-1 levels are associated with thrombotic events and is an established risk factor for cardiovascular diseases. The active conformation PAI-1 inhibits its target proteinases by the formation of a stable, inactive complex. Although PAI-1 is synthesized as an active molecule, it converts spontaneously to an inactive, latent form that can be partially reactivated by denaturing agents. In addition, a third conformation reacting as a non-inhibitory substrate towards various target proteinases has been identified.
The epitope of monoclonal antibody MA-124K1 is a conformational epitope close to the reactive-site loop. Glu220 and Glu212 constitute the main molecular determinants of the epitope for MA-124K1.
The monoclonal antibody MA-124K1 inhibits PAI-1 activity and simultaneously increases the binding of inactive PAI-1 to vitronectin. The effect of the antibody involves an allosteric modulation of the vitronectin binding site. The antibody cross reacts with mouse PAI-1.
Cross Reactivity
| Cross reactant |
Reactivity |
| Mouse |
Yes |
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The monoclonal antibody MA-124K1 can be used for immuno assays. Furthermore MA-124K1 is useful for inhibition of biological activity.
Use
For inhibition of biological activity dilutions have to be made according to the amounts PAI-1 to be inactivated.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Ngo, T et al; Identification of a target site in plasminogen activator inhibitor-1 that allows neutralization of its inhibitory properties concomitant with an allosteric up-regulation of its antiadhesive properties. J Biol Chem 2001, 276: 26243
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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