Technical datasheet
Description
The polyclonal antibody recognizes the human surfactant protein C (SP-C). There are four surfactant-specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant.
SP-C is a 34-35 amino acid peptide, of 4 kD that is proteolytically processed from a 21 kD precursor protein. SP-C is initiated early in the embryogenic period of lung formation, where SP-C transcripts are detected uniformly in epithelial cells lining the primitive airways. During lung development SP-C expression is decreased in cells of the proximal conducting portion of the lung. Ultimately SP-C is expressed selectively in type II epithelial cells in the alveolus of the lung. SP-C is secreted into the airspace where it enhances the spreading and stability of surfactant phospholipids in the alveolus. SP-C plays an important role in the spreading and stabilization of phospholipid films in the alveolus. SP-C is essential for air-breathing in mammals and is therefore largely conserved. Deficiency of SP-C and other surfactant components is associated with respiratory distress syndrome (RDS) in premature infants and adults with respiratory distress syndrome (ARDS).
The polyclonal antibody is raised against recombinant human pro-SP-C (AA 1-33), GST fusion. The polyclonal antibody is cross reactive with mouse SP-C.
Cross Reactivity
| Cross reactant |
Reactivity |
| Mouse |
Yes |
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Rabbit IgG
Application
The polyclonal antibody can be used for Western blotting. Furthermore the polyclonal antibody is useful for immunohystology on both frozen and paraffin sections.
Use
For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
SFTPC, previous SFTP2
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Ross, G etal; Surfactant protein C in fetal and ventilated preterm rabbit lungs. AM J Physiol Lung Cell Mol Physiol 1999, 277: 1104
- Nogee, L et al; Allelic heterogeneity in hereditary surfactant protein B (SP-B) deficiency. Am J Respir Crit Care Med 2000, 161: 973
- Nogee, L et al; A mutation in the surfactant protein C gene associated with familial interstitial lung disease. NEJM 2001, 344: 573
- Glasser, S et al; Altered stability of pulmonary surfactant in SP-C-deficient mice. PNAS 2001, 98: 6366
- Glasser, S, et al; Pneumonitis and emphysema in sp-C gene targeted mice. J Biol Chem 2001, 278: 14291
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
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