C1, Human, Natural - HC2122 - Hycult® Biotech

Name

C1, Human, Natural

Catalog nr

HC2122 (lot number and expiry date are indicated on the label)

Short description

Human complement protein C1 is the first component of the classical complement pathway. Activation of the C1-complex (composed of C1q, C1r and C1s, forming C1qr2s2), occurs when C1q binds to IgM or IgG complexed with antigens, or when C1q binds directly to the surface of the pathogen.
Activation of C1r and C1s occurs and so initiates the classical pathway of complement activation. The C1 activation products split C4 and then C2, producing C4b2a complex, which promotes cleavage of C3 into C3a and C3b.

Size

200 µg

Application

Technical datasheet

HC2122.pdf

Description

Cross Reactivity

Cross reactant	Reactivity
Trace amounts of C3, C4, or C5	< 0.1%

Formulation

1 ml of approximately 200 µg/ml purified natural human C1 isolated from healthy blood donors, in 50 mM NaAcetate, 50 mM EACA, 10 mM Benzamidine, 10 mM EDTA, 300 mM NaCl, 40% glycerol, pH 5.5. The blood donors have been tested and found negative for various viruses (see table: “Human blood test results”). The exact protein concentration and functional activity is indicated on the label.

Human blood test results
HBsAg	negative
HCV	negative
Anti-HIV 1 and 2	negative

Application

	F	FC	FS	IA	IF	IP	P	W
Yes			●
No
N.D.

.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot

Application notes

The biological activity of C1 is defined as the amount of C1 required to yield 50% lysis of sensitized erythrocytes (EA).

Use

For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Storage and stability

Product should be stored at –70°C. Repeated freeze and thaw cycles will cause loss of activity. The exact expiry date is indicated on the label. Use C1 protein within 24 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Also available

HC2123	C1q, Human, Natural
HC2124	C2, Human, Natural
HC2125	C3, Human, Natural
HC2128	C4, Human, Natural

MSDS

MSDS proteins and peptides.pdf

Related products		Cat #
C1q, Human, Natural		HC2123
C2, Human, Natural		HC2124
C3, Human, Natural		HC2125
C4, Human, Natural		HC2128