Technical datasheet
Description
C3 is the third component of the complement system and plays a central role in the complement cascade. The three pathways of compliment activation come together at this key molecule. Cleavage of the pivotal complement component C3 results in the generation of a set of effector molecules which have diverse biological functions. The cleavage product C3b promotes complement acrtivation and the subsequent formation of the membrane attack complex. C3a, the small (ca. 10 kDa) cleavage product possesses anaphylatoxic as well as immunoregulatory properties. It exerts its function through a specific receptor (C3aR), which belongs to the G-protein coupled receptor family. Expression of C3aR has been reported in many cell types including myeloid and non-myeloid cells. Expression of C3aR on haematopoietic stem/progenitor cells has been shown to promote the trafficking/homing of these cells to the BM. Engagement of C3aR on DCs and T cells has been shown to up-regulate these cell functions.
Formulation
1 ml of approximately 0.5 mg/ml purified natural human C3a isolated from healthy blood donors, in Phosphate buffered saline, pH 7.2.The blood donors have been tested and found negative for various viruses (see table: “Human blood test results”). The exact protein concentration and functional activity is indicated on the label.
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Human blood test results
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HBsAg
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negative
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Anti-HCV
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negative
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Anti-STS
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negative
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Anti-HIV 1 and 2
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negative
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Anti-HTLV-I/II
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negative
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Storage and stability
Product should be stored at –70°C. Repeated freeze and thaw cycles will cause loss of activity. The exact expiry date is indicated on the label. Use C3a protein within 24 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.
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