C4, Human, Natural - HC2128 - Hycult® Biotech

Name

C4, Human, Natural

Catalog nr

HC2128 (lot number and expiry date are indicated on the label)

Short description

The classical pathway of complement and the Mannose binding lectin activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved to result in C4c and C4d in which step all functional sites are lost. The monoclonal antibody 16D2 reacts with intact C4, C4b and C4d. C4 is an acute phase protein that is produced by hepatocytes, monocytes and intestinal epithelial cells.

Size

250 µg

Application

Technical datasheet

HC2128.pdf

Description

Cross Reactivity

Cross reactant	Reactivity
Trace amounts of functionally active C3	< 0.1%

Formulation

0.25 ml of approximately 1 mg/ml purified natural human C4 isolated from healthy blood donors, in Phosphate buffered saline, pH 7.2.The blood donors have been tested and found negative for various viruses (see table: “Human blood test results”). The exact protein concentration and functional activity is indicated on the label.

Human blood test results
HBsAg	negative
STS	negative
Anti-HTLV-1/II	negative
Anti-HCV	negative
Anti-HIV 1 and 2	negative

Application

	F	FC	FS	IA	IF	IP	P	W
Yes			●
No
N.D.

N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot

Application notes

The biological activity of C4 is defined as the amount of C4 required to yield 50% lysis of sensitized erythrocytes (EA).

Use

For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Storage and stability

Product should be stored at –70°C. Repeated freeze and thaw cycles will cause loss of activity. The exact expiry date is indicated on the label. Use C4 protein within 24 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Also available

HC2123	C1q, Human, Natural
HC2124	C2, Human, Natural
HC2125	C3, Human, Natural
HC2126	C3a, Human, Natural
HC2127	C3a desArg, Human, Natural
HC2128	C4, Human, Natural

MSDS

MSDS proteins and peptides.pdf

Related products		Cat #
C1q, Human, Natural		HC2123
C2, Human, Natural		HC2124
C3, Human, Natural		HC2125
C3a, Human, Natural		HC2126
C3a desArg, Human, Natural		HC2127
C4, Human, Natural		HC2128