Technical datasheet
Description
Monoclonal antibody M241 reacts with an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). Activated C1s is a glycosylated single-polypeptide zymogen of 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognised by the antibody M241 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M241 blocks C4 activation and C4 binding to activated C1s. The antibody binds specifically to the active centre of C1s.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS containing 0.1% bovine serum albumin.
Species
Mouse IgG2a
Application
The monoclonal antibody M241 can be used for immuno assays, Western blotting and immuno precipitation. Furthermore the monoclonal antibody M241 is useful for flow cytometry and immunohistology on frozen and paraffin sections. Monoclonal antibody M241 can be used for inhibition of biological activity.
Use
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity dilutions have to be made according to the amounts C1s to be inactivated.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Matsumoto, M et al; Probing the C4-binding site on C1s with monoclonal antibodies, evidence for a C4/C4b-binding site on the gamma-domain. J Immunol 1989, 8: 2743
- Nakagawa, K et al; Complement C1s activation in degenerating articular cartilage of rheumatoid arthritis patients: immunohistochemical studies with an active form specific antibody. Ann Rheum Dis 1999, 58: 175
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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