Technical datasheet
Description
The monoclonal antibody 755 recognizes an epitope located in the C-terminal 360 amino acids on the alpha chain of C3, thereby recognizing C3b and full C3. The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. An inherited deficiency of C3 predisposes a person to frequent bacterial infections. C3 fragments are deposited in tissues at sites of antibody-mediated immunopathology. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. After activation of the complement system, certain enzymes become active, resulting in the cleavage of C3 into C3b and the anaphylatoxin C3a. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. Within the alternative pathway of complement, C3b plays a critical role in the amplification loop initiated by spontaneous hydrolysis of C3.
Cross Reactivity
| Cross reactant |
Reactivity |
| Human C3a |
No |
Immunogen
Native C3
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG1
Application
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F
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FC
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FS1
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IA
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IF
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IP
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P
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W1,2
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Yes
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No
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N.D.
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application IA has been tested by Hycult Biotech.
Application notes
W: A non-reduced or reduced sample treatment and SDS-PAGE was used. The band size (s) are ~190 and ~100 kDa under non-reducing and reducing conditions respectively. (Ref.1)
Use
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Complement component 3
Positive control
Human serum
Negative control
C3 deficient serum
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
References
1. Klos, A et al; Detection of native human complement components C3 and C5 and their primary activation peptides C3a and C5a (anaphylatoxic peptides) by ELISAs with monoclonal antibodies. J Immunol Meth 1988, 111: 241
2. Hawlisch, H et al; Guinea pig C3 specific rabbit single chain Fv antibodies from bone marrow, spleen and blood derived phage libraries. J Immunol Meth 2000, 236: 117
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Also available
References
1. Klos, A et al; Detection of native human complement components C3 and C5 and their primary activation peptides C3a and C5a (anaphylatoxic peptides) by ELISAs with monoclonal antibodies. J Immunol Meth 1988, 111: 241
2. Hawlisch, H et al; Guinea pig C3 specific rabbit single chain Fv antibodies from bone marrow, spleen and blood derived phage libraries. J Immunol Meth 2000, 236: 117