Technical datasheet
Description
The monoclonal antibody 21B9 reacts with human CD21. CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kD protein. CD21 is a glycosylated type I transmembrane protein consisting on the extracellular face of a serie of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23-signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders The monoclonal antibody 21B9 reacts with CD21 on B-cells and dendritic cells. The monoclonal antibody 21B9 is a unique antibody which is also useful to recognize cells with low CD21 expression such as a subset of T cells, epithelial cells and astrocytes.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG3
Application
The monoclonal antibody 21B9 can be used for flow cytometry and Western blotting.
Use
For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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