Technical datasheet
Description
CD35 (CR1) is a type 1 membrane glycoprotein participating in clearance of immune complexes, regulation of autologous complement activation on cell surfaces, and complement-dependent cellular cytotoxicity mediated by leukocytes. CD35 inhibits complement activation by two distinct mechanisms. CD35 is a cofactor for factor I cleavage of C3b, iC3b and C4b. Furthermore CD35 mediates decay-dissociation of C3 and C5 convertases. CD35 is found on erythrocytes, neutrophils, monocytes and dendritic cells.
There are four allotypic variants of CD35 (CR1) with different molecular masses: 190 kDa (CR-1A), 220 kDa (CR1-B), 160 kDa (CR1-C) and 250 kDa (CR1-D).
The monoclonal antibody 31R binds a single epitope of CR1.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The monoclonal antibody 31R can be used for Western blotting, flow cytometry, immuno precipitation and immuno assay both as coating and detection antibody. Furthermore the monoclonal antibody 31R is useful for immuno purification and immunohistology on both paraffin and frozen sections.
Use
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Seya, T et al; Rapid purification of human complement receptor type 1 (CD35, CR1). J Biochem Biophys Methods 1996, 32: 69
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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