Technical datasheet
Description
The monoclonal antibody I8/1 recognizes human complement factor D. Factor D, a 27 kDa serine protease of the alternative complement pathway, is synthesized as a precursor single-chain molecule. The alternative complement pathway represents an important humoral component of natural defense against microbial attack. The participation of the alternative complement pathway has been implicated in the pathogenesis of a wide variety of human diseases.
Factor D is unique among serine proteases in that it requires neither enzymatic cleavage for expression of proteolytic activity nor activation by a serpin for its control. Factor D is highly specific and cleaves factor B bound to C3b, generating the C3bBb enzyme. Factor D is the rate-limiting C3 convertase enzyme of the alternative pathway. It has both the lowest plasma concentration and the lowest molecular weight of all complement components. Normal values in human EDTA plasma and urine are 1.05 (± 0.27) and 0.62 (± 0.33) µg/ml, respectively. In normal human subjects, factor D is rapidly eliminated via the kidney. Compared to controls, plasma and urine levels of factor D in patients with renal failure are elevated. Elevated plasma levels return to normal levels within seven days after successful renal transplantation to prevent overstimulation of the complement pathway.
Human adipsin, a protein that is expressed at high levels in adipose tissue, is identical to factor D and also displays the same enzymatic activity as factor D. This suggests that factor D plays a role in fatty tissue distinct from its role as a complement protein.
Immunogen
Serum purified factor D
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG1
Application
| | F | FC | FS | IA | IF | IP | P | W | | Yes | | | | ● | | | | | | No | | | | | | | | | | N.D. | ● | ● | ● | | ● | ● | ● | ● | |
N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot |
Use
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions.
Aliases
C3 convertase activator, Properdin factor D, Adipsin
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Oppermann, M et al; Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 1990, 133: 181
- Oppermann, M et al; Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int 1991, 40: 939
- Mavri, A et al; Impact of adipose tissue on plasma plasminogen activator inhibitor-1 in dieting obese women. Arterioscler Thromb Vasc Biol 1999, 19:1582
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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