Technical datasheet
Description
The monoclonal antibody MM10 recognizes membrane cofactor protein (MCP), also known as CD46. MCP is a type I membrane glycoprotein that consists of four isoforms i.e. two C-isoforms (51-58 kDa) and two BC isoforms (59-68 kDa) which arise by alternative splicing of a single CD46 gene. Membrane cofactor protein is expressed on every cell and tissue, with the exception of erythrocytes. Membrane cofactor protein serves to down-regulate the activation of complement on host tissue. MCP is a cofactor for factor 1-mediated cleavage of C3b and C4b and this way serves to protect the host cell against autologous attack. MCP is a receptor for several pathogens including measles virus, group A Streptococcus pyogenes and pathogenic Neisseria.
Cross Reactivity
| Cross reactant |
Reactivity (Yes/No) |
| Rat CD46 |
Yes |
Immunogen
Mouse CD46-human Fc fusion protein
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Rat IgG2a
Application
| | F2 | FC2 | FS | IA1 | IF2 | IP | P | W2 | | Yes | ● | ● | | ● | ● | | | ● | | No | | | | | | | | | | N.D. | | | ● | | | ● | ● | | |
N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot 1 Application Immuno assay has been tested by Hycult Biotech. 2.The following applications Flow cytometry, Immuno histochemistry with frozen sections, western blotting and immune fluorescence are based on original researcher data. |
Use
For immune histology, flow cytometry and western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilution. The typical starting working dilution is 1:50.
Aliases
MCP, CD46, Trophoblast leukocyte common antigen, TLX, measles virus receptor
Positive control
IA: Rat CD46 or mouse CD46 variant as coating on a micro titer plate
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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