Technical datasheet
Description
The monoclonal antibody TH9 recognizes rat CD59. Rat CD59 was previously known as rat inhibitory protein (RIP) and it is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 regulates the formation and function of the lytic C5b-9 complex by binding C8 and preventing the unfolding and membrane insertion of C9 and by binding C9 and restricting its polymerization.CD59 is a small (18 - 25 kDa) molecule, linked to the cell membrane through a glycosyl phosphatidylinositol (GPI) anchor and comprising 77 amino acids with a single N-linked carbohydrate group at Asn-18. Analogues of CD59 can be found in all species with similar structures and sizes.
In rat, CD59 is expressed on vacular endothelium and circulating cells. In the central nervous system (CNS) of the rat, CD59 is expressed on the Schwann sheath of peripheral nerve fibres and on ependymal cells, but not on glial cells and neurons in the CNS. Rat astrocytes in vitro express CD59 on its surface.
CD59 may be involved in rheumatoid arthritis, motor nerve injury in the Guillain-Barré sydrome and in other diseases where defective inhibition of complement activation on self tissue is involved. Furthermore, CD59 may play an important part in abrogating the effects of complement attack in renal disease. Its presence and protective effect have already been demonstrated on human renal cells.
Immunogen
Partially purified (FPLC) rat CD59 prep (RIP)
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
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F
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FC
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FS
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IA
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IF
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IP
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P
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W
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Yes
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No
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N.D.
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Use
For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
Aliases
Membrane attack complex inhibition factor, MACIF, MAC-inhibitory protein, Protectin, CD59
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Hughes, T et al; Isolation and characterization of a membrane protein from rat erythrocytes which inhibits lysis by the membrane attack complex of rat complement. Biochem J 1992, 284: 169
2. Hughes, T et al; Immunolocalization and characterization of the rat analogue of human CD59 in kidney and glomerular cells. Immunology. 1993, 80: 439
3. Rogers, C et al; Expression and function of membrane regulators of complement on rat astrocytes in culture. Immunology 1996, 88: 153
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Also available
References
1. Hughes, T et al; Isolation and characterization of a membrane protein from rat erythrocytes which inhibits lysis by the membrane attack complex of rat complement. Biochem J 1992, 284: 169
2. Hughes, T et al; Immunolocalization and characterization of the rat analogue of human CD59 in kidney and glomerular cells. Immunology. 1993, 80: 439
3. Rogers, C et al; Expression and function of membrane regulators of complement on rat astrocytes in culture. Immunology 1996, 88: 153