Technical datasheet
Description
The monoclonal antibody MM.1 recognizes CD46, also known as membrane cofactor protein. An immunohistological survey of rat tissues revealed that CD46 is expressed only in the testis and epididymis of adult rats more specifically, only by spermatozoa and late spermatozoal precursors.
In contrast, in humans, CD46 is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to down-regulate the activation of complement on host tissue. It functions as a cofactor by binding to C3b and C4b. Binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. In human, it also serves as a receptor for measles virus.
Two isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. The isoforms differ in the length. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)).
Immunogen
Second and third SCR domains of MCP fused to IgG-Fc; MCP23-Ig
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG1
Application
| | F1-3 | FC | FS | IA1 | IF2-4 | IP | P | W1,4 | | Yes | ● | | | ● | ● | | | ● | | No | | | | | | | | | | N.D. | | ● | ● | | | ● | ● | | |
N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot 1 Application immuno assay has been tested by Hycult Biotech. 1-4.The following applications Immunohistochemistry with frozen sections, western blotting and immunofluorescence are based on literature data and are not tested by Hycult Biotech. |
Use
For immunohistology, immunofluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Membrane cofactor protein
Positive control
Western blotting: rat testis lysate, non-reducing conditions, band size 38 kDa (Ref. 1and 4)
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Mizuno, M et al. Rat membrane cofactor protein (MCP; CD46) is expressed only in the acrosome of developing and mature spermatozoa and mediates binding to immobilized activated C3. Biol reprod 2004, 71: 1374
- Mizuno, M et al. Expression of CD46 in developing rat spermatozoa: ultrastructural localization and utility as a marker of the various stages of the seminiferous tubuli. Biol reprod 2005, 72: 908
- Mizuno, M et al. Spermatogenic cells distal to the blood-testis barrier in rats lack C3 convertase regulators and may be at risk of complement-mediated injury. J Reprod Immunol 2006, 69: 23
- Mizuno, M et al. CD55 in rat male reproductive tissue: differential expression in testis and expression of a unique truncated isoform on spermatozoa. Mol Immunol 2007, 44: 1613
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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