Technical datasheet
Description
The monoclonal antibody 8C8 recognizes human alpha-defensin 5 (HD5). HD5 is an antimicrobial peptide produced by Paneth cells, specialized epithelial cells of the small intestinal crypt. HD5 is stored in the Paneth cells as propeptide (aa 20-94) and released as mature peptide after trypsin digestion, which is co-secreted from the Paneth cell granules. The mature HD5 (aa 63-94) is protected from further digestion by the proteolysis enzyme. HD5 functions in maintaining the correct microbial balance in the intestine. Transgenic mice that express HD5 are protected from lethal challenge caused by Salmonella typhimurium. Expression levels of HD5 are negatively correlated to intestinal infection. HD5 is a useful marker for intestinal metaplasia of the upper gastrointestinal tract. HD5 expression has also been detected in the upper half of the stratified squamous epithelium of the vagina and ectocervix with increasing intensity toward the lumen. Secreted HD5 is detectable in cervicovaginal lavages, with highest concentrations during the secretory phase of the menstrual cycle. The monoclonal antibody 8C8 was raised against recombinant HD5 (aa 20-94) and recognizes the propeptide and the partially processed forms (aa 36-94 and 56-94), but it hardly recognizes mature peptide. No cross reactivity with HD6, lysozyme, or sPLA2 could be detected.
Formulation
0.5 mg of 0.2 μm filtered antibody solution in PBS (exact concentration is indicated on the label), containing 0.02% sodium azide.
Species
Mouse IgG2b
Application
The monoclonal antibody 8C8 can be used for immunoassays and Western blotting. Furthermore, the monoclonal antibody 8C8 is useful for immunohistology on frozen sections.
Use
For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
DEFA5
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Kelly, P et al; Reduced gene expression of intestinal alpha-defensins predicts diarrhea in a cohort of African adults. J Inf Dis 2006, 193: 1464
- Shen, B et al; Human defensin 5 expression in intestinal metaplasia of the upper gastrointestinal tract. J Clin Pathol 2005, 58: 687
- Kelly, P et al; Paneth cell granule depletion in the human small intestine under infective and nutritional stress. Clin Exp Immunol 2004, 135: 303
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
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