Technical datasheet
Description
Alpha defensins belong together with the ß-defensins to the family of cationic trisulfide-containing microbicidal peptides. They exert their microbicidal activity by permeating the membrane involving the formation of voltage regulated ion channels. Rabbit neutrophils contain large amount of alpha defensins (Neutrophil Defensin) NP 1 - 6. Defensins constitute more than 15% of total protein in rabbit neutrophils and are accumulated in dense cytoplasmic granules. Rabbit neutrophils express all six rabbit alpha-defensins, NP 1 - 6. Among myeloid rabbit alpha-defensins NP 1 - 6, NP 3 - 6 are neutrophil specific, while NP 1 - 2 are expressed in rabbit alveolar macrophages. Defensins are relatively resistant to proteolysis, low pH and boiling. Rabbit neutrophil defensins, whose three-dimensiol structures are very similar to those of human alpha defensins may be helpful in understanding the role of antimicrobial peptides in innate and acquired immunity and in the studies on the role of neutrophils in pathological situations and in various rabbit models of human diseases. Anti NP-5 antibody clone R3 recognizes natural NP-5 in biological solutions, in tissue sections and leukocyte smears fixed with ethanol, methanol/acetone or paraformaldehyde, in flow cytometry analysis of rabbit neutrophils stained by cell permeabilization method and in Western blotting reaction. Furthermore the antibody is capable to recognize synthetic and recombinant NP-5.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG2a
Application
The antibody can be used for NP-5 specific immuno assays, Western blotting, flow cytometry and immunohistological visualization of infiltrating neutrophils on frozen and paraffin sections.
Use
For flow cytometry, Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Hristova K, et al; Critical role of lipid composition in membrane permeabilization by rabbit neutrophil defensins. J Biol Chem 1997, 272: 24224
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
References
1. Hristova K, et al; Critical role of lipid composition in membrane permeabilization by rabbit neutrophil defensins. J Biol Chem 1997, 272: 24224