Technical datasheet
Description
The monoclonal antibody 66E2 recognizes human heart-type fatty acid-binding protein (H-FABP) of both natural and recombinant origin. The H-FABP protein is derived from the human FABP3 gene. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity that bind long chain fatty acids. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. H-FABP is localized in the heart, skeletal and smooth muscle, mammary epithelial cells, aorta, distal tubules of the kidney, lung, brain, placenta, and ovary. It is also useful as marker for brain damage. Furthermore, this antibody is useful for the purification of H-FABP.
Cross Reactivity
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Cross reactant
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Reactivity
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Rat H-FABP
Mouse H-FABP
Swine H-FABP
Human B-FABP
Human A-FABP
Human I-FABP
Human L-FABP
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Yes
Yes
Yes
Average (in ELISA)
No
No
No
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Immunogen
Purified Human H-FABP
Formulation
0.5 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG1
Application
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F
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FC
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FS
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IA1-4
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IF
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IP5
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P
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W3,4
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Yes
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●
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●
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●
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No
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N.D.
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●
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●
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●
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●
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●
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application IA and W have been tested by Hycult Biotech.
Application notes
IP: Biotinylated 66E2 was immobilized on streptavidin beads and added to serum to immunoprecipitate H-FABP (Ref.5).
W: Reduced sample treatment. The band size is ~15 kDa (Ref.4).
Use
For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Fatty acid-binding protein 3, FABP3, Muscle fatty acid-binding protein, Mammary-derived growth inhibitor
Positive control
Heart cells, or recombinant H-FABP
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Roos, W et al; Monoclonal antibodies to human heart fatty acid-binding protein. J Immunol Meth 1995, 183: 149
2. Guillaume, E et al; A potential cerebrospinal fluid and plasmatic marker for the diagnosis of Creutzfeldt-Jakob disease. Proteomics 2003, 3: 1495
3. Zimmermann-Ivol, C et al; Fatty acid binding protein as a serum marker for the early diagnosis of stroke. Mol Cell Proteomics 2004, 3: 66
4. Pelsers, M et al; Brain- and heart-type fatty acid-binding proteins in the brain: tissue distribution and clinical utility. Clin Chem 2004, 50: 1568
5. Zhen, E et al; Quantification of heart fatty acid binding protein as a biomarker for drug-induced cardiac and musculoskeletal necroses. Proteomics Clin Appl 2007, 1: 661
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Also available
References
1. Roos, W et al; Monoclonal antibodies to human heart fatty acid-binding protein. J Immunol Meth 1995, 183: 149
2. Guillaume, E et al; A potential cerebrospinal fluid and plasmatic marker for the diagnosis of Creutzfeldt-Jakob disease. Proteomics 2003, 3: 1495
3. Zimmermann-Ivol, C et al; Fatty acid binding protein as a serum marker for the early diagnosis of stroke. Mol Cell Proteomics 2004, 3: 66
4. Pelsers, M et al; Brain- and heart-type fatty acid-binding proteins in the brain: tissue distribution and clinical utility. Clin Chem 2004, 50: 1568
5. Zhen, E et al; Quantification of heart fatty acid binding protein as a biomarker for drug-induced cardiac and musculoskeletal necroses. Proteomics Clin Appl 2007, 1: 661