Technical datasheet
Description
The monoclonal antibody L2B10 recognizes human liver fatty acid binding protein (L-FABP) of both natural and recombinant origin. The L-FABP protein is derived from the human FABP1 gene. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity that bind long chain fatty acids. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. L-FABP is localized in the liver, kidney and intestinal epithelium. The monoclonal antibody L2B10 is useful to detect ischemic areas of human liver. Furthermore, the antibody can be used for the purification of human L-FABP.
Cross Reactivity
| Cross reactant |
Reactivity (Yes/No) |
| Baboon L-FABP |
Yes |
| Dog L-FABP |
Yes |
| Rat L-FABP |
Yes |
| Swine L-FABP |
Yes |
| Human H-FABP |
Weak |
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG2b
Application
| | F | FC | FS | IA | IF | IP | P | W | | Yes | ● | | | ● | | | | ● | | No | | | | | | | | | | N.D. | | ● | ● | | ● | ● | ● | | |
N.D. = Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot |
Use
For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
FABP1
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Bax, D et al; High-grade dysplasia in Barrett's esophagus is associated with increased expression of calgranulin A and B. Scand J Gastroenterology 2007, 42: 902
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
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