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C1q, Human, Natural

Catalog #: HC2123

Quantity: 1 mg

Availability: In stock

Description: C1q, Human, Natural

Human complement protein C1q forms together with C1r and C1s the C1 macromolecule, the first component of the classical complement pathway. Interaction of immune complexes with C1q induces a conformational change within the C1 complex, which results in activation of the classical pathway. C1q functions as recognition unit by binding to the heavy chain of IgG or IgM (Fc gamma and Fc micro) provided that the immunoglobulins are bound to their antigen. Furthermore, C1q can bind to apoptotic blebs, where it activates the classical complement pathway and mediates phagocytosis. As such, C1q promotes the clearance of apoptotic cells and subsequent exposure of auto antigens, thereby preventing stimulation of the immune system. C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. C1q deficiency has a profound effect on host defence and clearance of immune complexes. Absence of C1q may cause autoimmunity by impairment of the clearance of apoptotic cells. Inherited C1q deficiency is also associated with the development of systemic lupus erythematosus (SLE).

Specifications

Catalog number HC2123
Product type Proteins
Quantity 1 mg
Species Human
Formulation 1 ml of approximately 1 mg/ml purified natural human C1q isolated from healthy blood donors, in 10 mM HEPES, 300 mM NaCl, pH 7.2. The blood donors have been tested and found negative for various viruses (see table: Human blood test results). The exact protein concentration and functional activity is indicated on the label.
Use For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Application FS
Application Notes The biological activity of C1q is defined as the amount of C1q required to yield 50% lysis of sensitized erythrocytes (EA).
Storage and stability Product should be stored at -70 °C. Repeated freeze and thaw cycles will cause loss of activity. The exact expiry date is indicated on the label. Use C1q protein within 24 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease Nephrology, Neurological disorders