CD59a, Mouse, mAb 7A6

Catalog #: HM1116

Quantity: 100 µg

Availability: In stock

Description: CD59a, Mouse, mAb 7A6

The monoclonal antibody 7A6 (previously known as mCD59.3) recognizes mouse CD59a, a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 regulates the formation and function of the lytic C5b-9 complex by binding C8 and preventing the unfolding and membrane insertion of C9 and by binding C9 and restricting its polymerization.CD59 is a small (18 - 25 kDa) molecule, linked to the cell membrane through a glycosyl phosphatidylinositol (GPI) anchor and comprising 77 amino acids with a single N-linked carbohydrate group at Asn-18. Analogues of CD59 can be found in all species with similar structures and sizes. In contrast to all other species, mice have two genes encoding a CD59a and a CD59b protein. These two molecules are 63% identical at the amino acid level and share all major structural features. CD59a is broadly distributed on endothelia, erythrocytes, platelets and on numerous other cell types in organs, a distribution pattern resembling that of CD59 in other species. Expression of CD59b is restricted to germ cell elements in the testis and mature spermatozoa. Both CD59a and CD59b inhibit human and rodent complement with similar efficiency. CD59 may be involved in rheumatoid arthritis, motor nerve injury in the Guillain-Barré sydrome and in other diseases where defective inhibition of complement activation on self tissue is involved. Furthermore, CD59 may play an important part in abrogating the effects of complement attack in renal disease. Its presence and protective effect have already been demonstrated on human renal cells.

Specifications

Catalog number HM1116
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 µ/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Application Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno fluorescence, Western blot
Use For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Immunogen mCD59a-Fc
Isotype Mouse IgG1
Species Mouse
Alias Membrane attack complex inhibition factor, MACIF, MAC-inhibitory protein, Protectin, CD59
References 1. Harris, C et al; Characterization of the mouse analogues of CD59 using novel monoclonal antibodies: tissue distribution and functional comparison. Immunology 2003, 109: 117
2. Williams, A et al; Deletion of the gene encoding CD59a in mice increases disease severity in a murine model of rheumatoid arthritis. Arthritis Rheum 2004, 50: 3035
3. Willison, H et al; The role of complement and complement regulators in mediating motor nerve terminal injury in murine models of Guillain–Barré syndrome, J Neuroimmunol 2008, 201-202: 172
4. Yang, P et al; Expression and Modulation of RPE Cell Membrane Complement Regulatory Proteins. Invest Ophthalmol Vis Sci 2009, 50: 3473
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease Autoimmunity, Infectious diseases