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|Product description||Price||Quantity||Applications||Order #|
|LL-37/CAP-18, Human, mAb 3D11, functional study antibody, low endotoxin||
|0.5 mg||FS IF P W||HM2070-FS|
Description: LL-37/CAP-18, Human, mAb 3D11
|Product type||Monoclonal antibodies|
|Formulation||1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum.|
|Application||FS, IF, P, W|
|Application Notes||FS: Addition of the antibody reduces, after 3 h incubation, the cytotoxicity of D39 pneumococci induced by Human lung mast cells (HLMCs).
IF: Antibody was used on isolated neutrophils fixed with PMA on lysinated glass slides and blocked with PBS-5% goat serum-1% BSA.
P: Paraffin sections were deparaffinized, rehydrated and endogenous peroxidase was quenched using 0.3% H2O2 in methanol for 20 min. After antigen retrieval sections were incubated with 1.5 ug/ml antibody.
|Use||For immunohistology, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.|
|Immunogen||Human LL-37 peptide|
|Alias||Cathelicidin antimicrobial peptide, hCAP-18, Antibacterial protein LL-37, Cap-18, CRAMP; HSD26; FALL39; FALL-39; CAMP|
|References||1. Sørensen, O et al; Human cathelicidin, hCAP-18, is processed to the antimicrobial peptide LL-37 by
extracellular cleavage with proteinase 3. Blood 2001, 97: 3951
2. Nell, M et al; Bacterial products increase expression of the human cathelicidin hCAP-18/LL-37 in cultured human sinus epithelial cells. FEMS Immunol Med Microbiol 2004, 42: 225
3. Kessenbrock, K et al; Netting neutrophils in autoimmune small-vessel vasculitis. Nature med 2009, 15: 623
4. Cruse, G et al; Human lung mast cells mediate pneumococcal cell death in response to activation by pneumolysin. J Immunol 2010, 184: 7108
5. Coffelt, S et al; The pro-inflammatory peptide LL-31 promotes ovarian tumor progression through recruitment of multipotent mesenchymal stromal cells. PNAS 2009, 106(10): p3806-3811
|Storage and stability||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
|Disease||Autoimmunity, Infectious diseases|