C3a/C3a des Arg, Human, mAb 2991

Catalog #: HM2074

Quantity: 100 µg

Availability: In stock

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C3a/C3a des Arg, Human, mAb 2991, immuno assay antibody 0.5 mg IA W HM2074-IA Find distributor

Description: C3a/C3a des Arg, Human, mAb 2991

The monoclonal antibody 2991 recognizes the neo-epitope of human C3a/C3a-desArg that is formed upon cleavage of the complement protein C3 into C3a and C3b. The complement system is an important part of the humoral response in innate immunity, consisting of three different pathways. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. Monoclonal antibodies detecting neo-epitopes have been used for direct quantification of activation at different steps in the complement cascade. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. In ulcerative colitis, and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. Proteolysis by certain enzymes results in the cleavage of C3 releasing C3a anaphylatoxin and C3b. C3a is a protein of 74 amino acids. C3a itself is very short-lived and in serum is cleaved rapidly into the more stable C3a-desArg (also called acylation stimulating protein, ASP) . Therefore, measurement of C3a-desArg allows reliable conclusions about the level of complement activation in the test samples. C3a is a mediator of local inflammatory processes. It induces smooth muscle contraction, increases vascular permeability, and causes histamine release from mast cells and basophilic leukocytes. C3a is involved in inflammatory reactions seen in gram-negative bacterial sepsis, trauma, ischemic heart disease, post-dialysis syndrome and a variety of autoimmune diseases. Normal values in plasma of control persons range between 48 150 ng/ml (median: 86.4 ng/ml, SD: 29.3 ng/ml)
Monoclonal antibody 2991 recognizes both C3a and C3a-desArg, with at least a 5x higher preference for C3a-desArg.

Specifications

Catalog number HM2074
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Application Immuno assays, Western blot
Application Notes W: A reduced sample treatment and SDS-PAGE was used. The band size is ~8 kDa.
Use Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Immunogen Human C3a desArg
Isotype Mouse IgG1
Species Human
Alias Acylation stimulating protein, ASP
Gene name: C3
Positive Control Activated human serum or purified human C3a desArg protein
Negative Control For Western blot, any irrelevant protein can be used.
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Infectious diseases, Nephrology