CD59, Human, mAb MEM-43

Catalog #: HM2120

Quantity: 100 µg

Availability: In stock

Description: CD59, Human, mAb MEM-43

The antibody is the prototypic antibody for the CD59 determinant, a PI-linked glycoprotein of 18-25 kDa, which is broadly expressed on most types of cells. The antigen is the major complement-protecting molecule of human cells. It is expressed on all leukocytes, erythrocytes and most other cell types. Binding of this antibody inhibits the complement-protecting activity of CD59. CD59 is one of the complement regulatory membrane proteins that is missing in Paroxysmal Nocturnal Haemoglobinuria (PNH). Anti CD59 reagents may be useful in the differential diagnosis of PNH type II and PNH type I. Recently a role for CD59 in LPS signal transduction has been reported in keratinocytes. The antibody reacts with well defined epitope (W40, R-53) on CD59 molecule.

Specifications

Catalog number HM2120
Product type Monoclonal antibodies
Quantity 100 µg
Formulation Sterile 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS and 0.1% bovine serum albumin with low endotoxin level.
Application Flow cytometry, Functional studies, Immuno precipitation, Western blot
Use For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts endotoxin to be inactivated.
Isotype Mouse IgG2a
Species Human
References 1. Yakamoto, T et al; Lipopolysaccharide signal transduction in oral keratinocytes - involvement of CD59 but not CD14. Cellular Signalling 2003, 15: 861
2. Horesji, V et al; Folia Biol 1986, 34: 12
3. Knapp, W et al; Leucocyte Typing IV, Oxford University Press 1989
4. Schlossman, S et al; Leucocyte Typing IV, Oxford University Press 1995
5. Cebecauer, M et al; Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun 1998, 234: 1998
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Autoimmunity, Infectious diseases